Vol 20, No 4 (2014)

In 2010-2012, the primary data of bio-impedance measurements in 2 092 695 patients in 484 health centers was used to evaluate prevalence of obesity and surplus body mass in population of Russia depending on age and gender by WHO criteria. The standardized rate of occurrence of obesity in children and adolescents aged 5-17 years old made to 6.8% for males and 5.3% for females and 21.9% and 29.7% in adults correspondingly. The localized criteria of bio-impedance diagnostic of obesity according percent content of fat in body mass was developed. The low diagnostic sensitivity (50% for males and 65% for females) and high specificity (90%) of WHO criterion of obesity diagnostic according body mass index was revealed.

The article presents data of comparative study of level in blood of C-reactive protein, a number of other proteins of acute phase of inflammation response of organism (haptoglobin, a1-antitrypsin), marker of tissue destruction of myoglobin and activity of certain enzymes of energy metabolism (creatine kinase, alkaline phosphatase, lactate dehydrogenase) in the course of dynamic observation of course of malignant process in 42 patients with sarcoma of soft tissues. The possibility of implementation of examined indicators as biochemical criteria of generalization of process was analyzed. It is demonstrated that under monitoring of patients with sarcoma of soft tissues the most prognostic information value is attached to detection of C-reactive protein. The clinical examples of application of this indicator to forecast the course of disease in patients with sarcoma of soft tissues.

The article presents results of comparative analysis of levels of IgG-antibodies in serums of women who are positive to simple herpes virus. All female respondents were of reproductive age and were divided in two samplings i.e. women up to 12 weeks of pregnancy (n = 89) and women with barrenness in marriage (n = 123). In infertile female patients and pregnant women the virus-specific antibodies and their avidity were detected under simultaneous analysis of serums using method of enzyme-linked immunosorbent assay. The concentration of antibodies was expressed min coefficients of positivity and in titers. The mean value of concentration of serum IgG-antibodies in female patients with barrenness was statistically higher (coefficient of positivity = 6.67 ± 3.48; titer 1:800-1:3200) than in pregnant women (coefficient of positivity = 2.29 ± 1.48; titer 1:100, p = 0.001). The analysis of content of antiviral antibodies in female patients with barrenness depending on effectiveness of programs of extracorporal fertilization demonstrated that highest rate of pregnancy onset (36.1%, 30/83) was observed in female patients with low level of antibodies (n = 83, coefficient of positivity = 3.48 ± 2.4; titer 1:800). Whereas, in female patients with high level of IgG-antibodies to simple herpes virus (n = 40, coefficient of positivity = 10.0 ± 2.05; titer 1:3200) this indicator consisted 17.5% (7/40, p = 0.039). The supposition was made that high level of IgG-antibodies to simple herpes virus is associated with frequent episodes of productive herpes viral infection. To confirm this assumption the experiments with lab animals were organized. The evaluation was made of dynamics of levels of IgG-antibodies in serum of pubertal females mice DBA/2J infected with reference-strain L2 of simple herpes virus type I intravaginally in two groups. Virus was introduced to animals from first group (n = 14) once and to animals from second group (n = 14) twice with interval of 4 weeks. The concentration of antibodies was evaluated using method of enzyme-linked immunosorbent assay with application of pair serums of animals with twice outtake of blood after every 4 weeks after virus inoculation. Out of 28 animals of both groups 6 mice (22%) were seronegative (no detection of antibodies in whole serum), 22 mice (78%) were seropositive (coefficient of positivity > 1.0, titer 1:50). Out of 22 immune mice only 4 female mice (19%) had expressed antiviral immune response (coefficient of positivity > 4.0) whereas in 18 out of 22 mice (81%) level of synthesis of mentioned immunoglobulins was significantly lower (coefficient of positivity = 1.7 ± 0.41). The increase of concentration of IgG-antibodies occurred only in the group with two-time introduction of simple herpes virus (titer 1:200- 1:400). The content of serum antibodies in female mice with single infection had no changes in course of time. These data indicates that in overwhelming majority of cases increase of level of IgG-antibodies is conditioned by synthesis of viral antigens de novo. Summing up obtained results makes it possible to conclude that high concentration of IgG-antibodies to simple herpes virus testifies productive herpes viral infection and has greater diagnostic value than it considered before. The fact that level of mentioned antibodies is statistically higher in sampling of women with barrenness indicates the negative effect of virus on female reproductive system.

The results of study testify that in patients the diabetes mellitus type I is accompanied by dyslipidaemia of atherogenic character. The activation of processes of lipid peroxidation is also present and results in increasing of content of both primary and secondary products of peroxidation of lipids and disorders in system of antioxidant defense. In patients with diabetes mellitus increase of total content of saturated fatty acids mainly at the expense of myristinic acid accompanied by decreasing of total content of unsaturated fatty acids. This occurrence was mostly expressed in patients with complications of diabetes mellitus. The study marked direct correlation relationship between activity of superoxide dismutase and level of a-linolenic acid (r=0.53, p<0.05), and also between level of diene conjugates and enzyme activity of glutathione peroxidase (r=0.47, _p<0.05). The direct correlation relationship existed between concentration of malondialdehyde and content of linolenic acid (r=0.67, p<0.05). The total ratio of content of omega-6/omega-3 decreased (p<0.05) at the expense of increase of total activity of superoxide dismutase and catalase (r=-0.763, p<0.05). Therefore, in patients with complicated diabetes mellitus against the background of dyslipidaemia significant alterations were established concerning both level and content of fatty acids, rising of concentration of free radicals and decrease of activity of enzymes of antioxidant defense.

The provocation broncho-constricting tests are largely applied all over the world with the purpose of diagnostic and differentiated diagnostic of bronchial asthma and chronic obstructive disease of lungs and control of effectiveness of applied therapy. The review presents actual data concerning application of provocation tests in pulmonology.

Nowadays, problem of inconsistency of neurorrhaphy is actual. The results of nerve suturing depend on preservation of possibility of regeneration, degree of further cicatrization, intention of zone of anastomosis, precision of approximation of fascicles. Lately, various options of stimulation of axonal growth are applied more broadly. Among them are direct combined electrostimulation of peripheral nerves, in aggregate with mechanotherapy and introduction of anticholinesterase preparations. In the academician G.A. Ilizarov Russian research center “The restorative traumatology and orthopedic” different versions of electrostimulation of peripheral nerves are developed and implemented. The mode of neurorrhaphy enabling exact coaptation of fascicles groups. This mode can be recommended to be applied in neurosurgery and traumotology departments and under microsurgery reconstructive interventions on damaged peripheral nerves.