Formation of Bone and Foci of Ectopic Hemopoiesis at Joint Application of Calcium Scaffolds with Bone Marrow Cells or Cultivated Mesenchymal Stromal Cells


Cite item

Full Text

Abstract

Potential application of calcium scaffolds in combination with either bone marrow or adherent cell layers from long-term cultures containing mesenchymal stem cell for the induction of bone tissue growth was studied in mice. Two scaffolds, i.e. Osteoset® and Prodens® were tested on the model of ectopic grafting under the skin and renal capsule of mice. It was demonstrated that Prodens® and less effectively Osteoset® could be used for the induction of bone growth in combination with bone marrow cells but even more effectively in combination with cultivated mesenchymal stromal cells. Both the site of transplantation and preliminary induction of bone differentiation of stromal cells exerted a great influence upon the process of bone formation.

About the authors

Irina Nikolaevna Shipunova

ГНЦ РАМН

Email: iranifontova@yandex.ru
анд. биол. наук, науч. сотр. лаборатории физиологии кроветворения; ГНЦ РАМН

D A Svinareva

ГНЦ

канд. биол. наук, науч. сотр. той же лаборатории; ГНЦ

T V Petrova

ГНЦ

аспирант той же лаборатории; ГНЦ

M M Ryashentsev

ГНЦ

канд. мед. наук, старший науч. сотр. отделения реконструктивно-восстановительной хирургии для больных гемофилией; ГНЦ

V E Mamonov

ГНЦ

канд. мед. наук, науч. руководитель того же отделения; ГНЦ

N I Drize

ГНЦ

профессор, доктор биол. наук, зав. лабораторией физиологии кроветворения; ГНЦ

I N Shipunova

D A Svinaryova

T V Petrova

M M Ryashentsev

V E Mamonov

N I Drize

References

  1. Чертков И.Л., Гуревич О.А. Стволовая кроветворная клетка и ее микроокружение. - М., 1984.
  2. Bruder S.P., Kraus K.Y., Goldberg V.M., Kadiyala S. The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects //J. Bone Jt Surg. - 1998. - Vol. 80A. - P. 985-996.
  3. Caplan A.I. Mesenchymal stem cells //J. Orthop. Res. -1991. - N 9. - P. 641-650.
  4. Damien C.J., Parsons J.R. Bone graft and bone graft substitutes: a review of current technology and applications //J. Appl. Biomater. - 1991. - N 2. - P. 187-208.
  5. Dexter T.M., Allen T.D., Lajtha L.G. Conditions controlling the proliferation of haemopoietic stem cells in vitro //J. Cell Physiol. - 1977. - Vol. 91. - P. 335-344.
  6. Dinopoulos H.T., Giannoudis P.V. Safety and efficacy of use of demineralised bone matrix in orthopaedic and trauma surgery //Expert. Opin. Drug Saf. - 2006. - N 5. - P. 847-866.
  7. Drosos G.I., Kazakos K.I., Kouzoumpasis P., Verettas D.A. Safety and efficacy of commercially available demineralised bone matrix preparations: a critical review of clinical studies //Injury. - 2007. - Vol. 38, Suppl 4. - P. S13-S21.
  8. Grundel R.E., Chapman M.W., Yee T., Moore D.C. Autogeneic bone marrow and porous biphasic calcium phosphate ceramic for segmental bone defects in the canine ulna //Clin. Orthop. - 1991. - N 266 . - P. 244-258.
  9. Gurevitch O., Kurkalli B.G., Prigozhina T. et al. Reconstruction of cartilage, bone, and hematopoietic microenvironment with demineralized bone matrix and bone marrow cells //Stem Cells. - 2003. - Vol. 21. - P. 588-597.
  10. Petite H., Viateau V., Bensaid W. et al. Tissue-engineered bone regeneration //Nat. Biotechnol. - 2000. - Vol. 18. - P. 959-963.
  11. Prockop D.J. Marrow stromal cells as stem cells for nonhematopoietic tissues //Science. - 1997. - Vol. 276. - P. 71-74.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2009 Eco-Vector



Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).