The use of real-time PCR for study of the periodontal microbiome in patients with combined pathology of the gastroduodenal zone and chronic periodontitis

Abstract

A total of 54 patients with chronic periodontitis of differing severity was tested using real-time PCR (Dentoflor kit). The group included 38 patients with chronic gastritis. For the first time, a higher prevalence of Treponema denticola in periodontium of males in comparison with females was demonstrated. The patients with chronic gastritis had more human genome DNA at their periodontium than healthy individuals. Nonparametric statistical analysis demonstrated high association of periodontium colonization with T. forsythensis and T. denticola (but not Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia) with the severity of the chronic periodontitis.