Development of a method for identification and genotyping of Pasteurella multocida and Mannheimia haemolytica bacteria using polymerase chain reaction and phylogenetic analysis of bacterial cultures isolated from cattle

Abstract

The results of developing the identification and genotyping method for the Pasteurella multocida bacteria of five capsule groups and the Mannheimia haemolytica A 1 bacteria, using the multiplex polymerase chain reaction (PCR) with the electrophoretic detection are reported. The diagnostic sensitivity of the developed method came to 10³ CFU/mL in the pure culture studies and 10⁵ CFU/g in the biological material studies. The analysis revealed 50% of P. multocida and 11.2% of M. haemolytica in all 260 tested samples of biological material from the infected animals. Circulation of bacteria of P. multocida capsule groups B and E among the susceptible animals was not determined. Group A bacteria were found in the majority of the samples; bacteria of group D were infrequently identified; in one case, group F bacteria were detected. The circulation of capsule group A P. multocida bacteria of two genetic types was determined using phylogenetic analysis.