Characteristics of fungal strains producing thermostable xyloglucanases from the Russian National Collection of industrial microorganisms

Abstract

Fungal strains possessing xyloglucanase activity have been selected from the microorganisms degrading plant biomass deposited in the Russian National Collection of Industrial Microorganisms (VKPM). The thermophilic strains Sporotrichum thermophile VKPM F-972, Myceliophthora thermophila VKPM F-244, and Sporotrichum pruinosum VKPM F-235 produced extracellular xyloglucanases under conditions of submerged cultivation with optimum temperature of 60°C and pH of 5.0. The enzymes retained 88–100 and 79–84% of the initial activity after 1-h incubation at 50° and 60°C, respectively. Thus, strains S. thermophile VKPM F-972, M. thermophila VKPM F-244, and S. pruinosum VKPM F-235 may be used as sources of genes for construction of highly active producers of thermostable xyloglucanases.