Email this article Quantitative Analysis of the Microbiota of Periodontal Pockets and Saliva by Real-Time PCR before and after Treatment of Periodontitis
- Authors: Baymiev A.K.1,2, Shvec K.Y.1, Mavzjutov A.R.1, Tamarova J.R.1, Bulgakova A.I.1
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Affiliations:
- Bashkir State Medical University
- Institute of Biochemistry and Genetics, Ufa Scientific Center
- Issue: Vol 32, No 3 (2017)
- Pages: 155-159
- Section: Experimental Works
- URL: https://journal-vniispk.ru/0891-4168/article/view/178207
- DOI: https://doi.org/10.3103/S0891416817030028
- ID: 178207
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Abstract
Work has been done on standardization of detection and quantification of periodontopathogenic microorganisms in the clinical material (contents of periodontal pockets and saliva) using real-time PCR. To optimize the conditions for the analysis, a method for obtaining clinical samples of known volume was developed and a calibration sample to obtain reliable results in the diagnosis of periodontitis was designed.
About the authors
Al. Kh. Baymiev
Bashkir State Medical University; Institute of Biochemistry and Genetics, Ufa Scientific Center
Author for correspondence.
Email: baymiev@mail.ru
Russian Federation, Ufa, 450008; Ufa, 450054
K. Yu. Shvec
Bashkir State Medical University
Email: baymiev@mail.ru
Russian Federation, Ufa, 450008
A. R. Mavzjutov
Bashkir State Medical University
Email: baymiev@mail.ru
Russian Federation, Ufa, 450008
Je. R. Tamarova
Bashkir State Medical University
Email: baymiev@mail.ru
Russian Federation, Ufa, 450008
A. I. Bulgakova
Bashkir State Medical University
Email: baymiev@mail.ru
Russian Federation, Ufa, 450008
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