Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing

Abstract

For the rapid preparation of strains of live influenza vaccine, it is important to have a rapid method for screening vaccine reassortants. In this paper, a method of pyrosequencing was used to analyze the genome of reassortants obtained by crossing the donor of attenuation B/Leningrad/14/17/55 and the wild-type virus B/Indiana/25/2015. It has been shown that pyrosequencing allows the composition of the reassortant genome to be evaluated quickly, economically, and with high accuracy.