Frequency of Canine Parvovirus Type-2 Variants by PCR in Enteric and Healthy Dogs and Genotyping of Variants by Restriction Enzyme (RE) Mapping by DdeI Endonuclease Based on Partial VP-2 Gene Sequence

Abstract

Canine parvovirus 2 (CPV-2) is the causative agent of myocarditis and acute hemorrhagic enteritis in dog populations. In this study, vaccinal strains and sixty wild strains (CPV-2a, CPV-2b) of CPV-2 from fecal samples of enteric (30 strains) and healthy (30 strains) dogs detected by Duplex-PCR, were compared together by application of RE mapping based on a partial sequence of VP2 gene (681 bp) by DdeI endonuclease. Our results showed that both CPV-2a and CPV-2b are pathogenic for dogs, but CPV-2a are more prevalent in healthy dogs while CPV-2b strains are more prevalent in enteric cases, significantly (p < 0.05). RE mapping based on DdeI restriction endonuclease demonstrated homology in the nucleotide sequence in amplified part of VP2 gene region in the genome of vaccinal and wild strains of CPV-2 detected in fecal samples of tested animals.