Email this article Identification of Markers for Dengue Infection in Patients after Visiting Dengue-Endemic Countries
- Authors: Ternovoi V.A.1, Plyasunova I.V.1, Sementsova A.O.1, Kartashov M.Y.1, Shvalov A.N.1, Chausov E.V.1, Eremeeva L.I.1, Protopopova E.V.1, Chub E.V.1, Bayandin R.B.1, Pyankov O.V.1, Loktev V.B.1, Agafonov A.P.1, Maksyutov R.A.1
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Affiliations:
- State Research Center of Virology and Biotechnology VECTOR
- Issue: Vol 34, No 3 (2019)
- Pages: 188-194
- Section: Experimental Works
- URL: https://journal-vniispk.ru/0891-4168/article/view/178382
- DOI: https://doi.org/10.3103/S0891416819030066
- ID: 178382
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Abstract
In studied blood serum samples from Russian patients hospitalized with suspected dengue fever, one or more markers of dengue fever have been detected, namely, viral RNA, NS1 antigen, and IgM or IgG antibodies. Moreover, only the combined use of various immunological and genetic methods provided a more effective diagnosis of dengue fever. The detection of IgG to dengue virus in patients with high titers of IgG to tick-borne encephalitis virus (TBEV) and, conversely, the detection of IgG to TBEV virus in patients with high titers of IgG to dengue has been documented. The results confirm the literature data on pronounced antigenic cross-sections between different representatives of flaviviruses, which may complicate the ELISA diagnosis of TBEV and dengue in patients. Genotyping of obtained dengue virus isolates showed that imported cases of dengue fever are caused by subtypes 1–4 of the dengue virus, with the prevalence of infection cases caused by dengue virus 1. Sequencing of these dengue virus isolates, including genome-wide, showed that viral isolates obtained on the territory of Russia are phylogenetically close to modern variants of the dengue virus circulating in the countries of Southeast Asia.
About the authors
V. A. Ternovoi
State Research Center of Virology and Biotechnology VECTOR
Author for correspondence.
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
I. V. Plyasunova
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
A. O. Sementsova
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
M. Yu. Kartashov
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
A. N. Shvalov
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
E. V. Chausov
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
L. I. Eremeeva
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
E. V. Protopopova
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
E. V. Chub
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
R. B. Bayandin
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
O. V. Pyankov
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
V. B. Loktev
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
A. P. Agafonov
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
R. A. Maksyutov
State Research Center of Virology and Biotechnology VECTOR
Email: tern@vector.nsc.ru
Russian Federation, Novosibirsk oblast, 630559
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