Imaging Doxorubicin Free Radical in Mice with Overhauser Enhanced MRI and its Tumor Suppression Effect in Mice

Abstract

In the treatment with anticancer drugs, it is important to deliver an anticancer agent to target site of the tumor at an appropriate concentration. However, it is difficult to directly measure the distribution amount of the agent and effect of anticancer drug is evaluated using its tumor suppression effect. In this study, we report an approach to visualizing an anticancer agent distribution in tumor-bearing mouse model using Overhauser enhanced magnetic resonance imaging (OMRI). The agent, doxorubicin, is one of anthracycline anticancer drugs and can form a free radical at its quinone sites and could be visualized using OMRI. After direct injection into a tumor, doxorubicin free radical was successfully imaged in tumor-bearing mouse, demonstrating practical usefulness of OMRI in the study of pharmacodynamics of free radical compounds. Imaging of antitumor agent would be potentially useful as a guidance tool for image-guided-therapy of cancer local chemotherapy.