Phytochelatin synthesis and responses of antioxidants during arsenic stress in Nasturtium officinale

Abstract

The induction of thiols including glutathione and phytochelatins as well as the activities of antioxidant enzymes namely superoxide dismutase isoenzymes and those enzymes involved in the ascorbate-glutathione cycle in watercress plants under arsenic stress were investigated. Arsenic concentrations and tissue type-dependent response to arsenic were assessed. Plant was capable of accumulating large amounts of arsenic in the shoots. Superoxide dismutase isoenzymes activity and phytochelatin level was higher in shoots than in roots. In roots, ascorbate levels increased significantly, while no relationship was found between ascorbate contents and arsenic tolerance in shoots. Treatment with arsenic resulted in a remarkable increase in glutathione content of roots at all of the arsenic concentrations, while in shoots, glutathione content increased by lower levels of arsenic. Differences were noted in both roots and shoots for enzymes involved in the ascorbate-glutathione cycle. These results suggest that, the strategy of tolerance to arsenic toxicity in roots of watercress plants is different from that of shoots.