Differential Induction of β-1,3-Glucanase Gene in Expression of Partial Resistance to Rust (Uromyces fabae (Pers.) de-Bary) in Pea (Pisum sativum L.)
- Authors: Kushwaha C.1, Chand R.2, Singh A.K.3, Kumar M.2, Srivastava C.P.4
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Affiliations:
- Department of Plant Pathology
- Department of Mycology and Plant Pathology, Institute of Agricultural Sciences
- Department of Genetics and Plant Breeding
- Department of Genetics and Plant Breeding, Institute of Agricultural Sciences
- Issue: Vol 65, No 5 (2018)
- Pages: 697-701
- Section: Research Papers
- URL: https://journal-vniispk.ru/1021-4437/article/view/180197
- DOI: https://doi.org/10.1134/S1021443718050114
- ID: 180197
Cite item
Abstract
The present study highlights differential induction of pathogenesis related protein PR-2 (β-1,3- glucanases) in expression of rust resistance in pea using different resistant and susceptible recombinant inbred lines of pea (Pisum sativum L.). The enhanced levels of glucanase expression was noted in resistant genotypes at 24 h post inoculations that was negatively correlated (–0.54) with Area Under Disease Progress Curve (AUDPC) and positively correlated (0.67) with lignin accumulations. A significant role of structural defence mechanism in rust resistance in pea was evident from reduced colony size and lesser number of haustorium per colony in resistant lines as well as their negative correlations with lignin accumulation and AUDPC. Gene specific markers indicated constitutive nature of glucanase and peroxidase genes in test genotypes, though differential expression of the glucanase activity was observed in the resistant and susceptible genotypes. However, association of peroxidases with resistance to pea rust is yet to be established due to its non-specific role in slow rusting in pea. The result showed a significant role of β-1,3-glucanase in expression of rust resistance in pea.
Keywords
About the authors
C. Kushwaha
Department of Plant Pathology
Author for correspondence.
Email: rc_vns@gmail.com
India, Sabour, Bhagalpur
R. Chand
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences
Email: rc_vns@gmail.com
India, Varanasi
A. K. Singh
Department of Genetics and Plant Breeding
Email: rc_vns@gmail.com
India, Chhattisgarh
M. Kumar
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences
Email: rc_vns@gmail.com
India, Varanasi
C. P. Srivastava
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences
Email: rc_vns@gmail.com
India, Varanasi
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