Email this article Sucrose Synthase (SuSy) Gene Expression: An Indicator for Cotton Fiber Initiation and Early Development
- Authors: Ahmed M.1,2, Akhtar S.2, Fanglu M.1, Hasan M.M.2, Shahid A.A.2, Yanang X.2, Sarwar M.B.2, Rao A.Q.2, Husnain T.2, Wang X.1
-
Affiliations:
- Institute of Crop Sciences, College of Agriculture and Biotechnology, Faculty of Agriculture Life and Environment Sciences Zhejiang University
- National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
- Issue: Vol 66, No 1 (2019)
- Pages: 41-49
- Section: Research Papers
- URL: https://journal-vniispk.ru/1021-4437/article/view/180314
- DOI: https://doi.org/10.1134/S1021443719010023
- ID: 180314
Cite item
Open Access
Access granted
Subscription Access
Abstract
Cotton (Gossypium hirsutum L.) fiber initiation from ovule epidermal cells happen from 0 to 5 DPA, invertase (INV) and sucrose synthase (SuSy) are crucial for fiber initiation, cell expansion, and elongation. We used two commercial cotton varieties, Xuzhou 142-normal wild-type (WT) and Sea Island PimaS-4 (PimaS-4) as controls and compared with three fiber mutants, Xuzhou 142-fl (fl), Xuzhou 142-N (N) and Ligon lintless (Li). SuSy, INV activity, sugars and malate content were measured at fiber cell initiation and early development stage. Increased Susy activity was detected in WT, PimaS-4 and Li ovules at 0 DPA than the fiber mutant lines fl and N. On the other hand, fl mutant showed high sucrose contents than N and Li during 0 to 1 DPA. No significant changes happen in studied cotton lines with respect to INV from 1 to 5 DPA altogether. There was a significant difference in total soluble sugars and malate contents between WT and fl cotton ovules at early elongation stage (5 DPA). The results revealed that SuSy activity at anthesis day indicates the fate of ovule epidermal cells to bulge out and form fiber initials. The reduced SuSy activity in fl ovules at 0 DPA results in a lack of fiber cell initiation and lead to fiberless seed phenotype. The study will pave the way towards unraveling the mechanism of fiber initiation and development by exploring the role of different fiber-related genes.
About the authors
M. Ahmed
Institute of Crop Sciences, College of Agriculture and Biotechnology, Faculty of Agriculture Life and Environment Sciences Zhejiang University; National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
China, Hangzhou, 310058; Lahore, 53700
S. Akhtar
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
M. Fanglu
Institute of Crop Sciences, College of Agriculture and Biotechnology, Faculty of Agriculture Life and Environment Sciences Zhejiang University
Email: xdwang@zju.edu.cn
China, Hangzhou, 310058
M. M. Hasan
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
A. A. Shahid
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
X. Yanang
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
M. B. Sarwar
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
A. Q. Rao
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
T. Husnain
National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab Lahore
Email: xdwang@zju.edu.cn
Pakistan, Lahore, 53700
X. Wang
Institute of Crop Sciences, College of Agriculture and Biotechnology, Faculty of Agriculture Life and Environment Sciences Zhejiang University
Author for correspondence.
Email: xdwang@zju.edu.cn
China, Hangzhou, 310058
Supplementary files
