Rapid Visual Detection of Four Specific Transgenic Events in GM Soybean Using Loop-Mediated Isothermal Amplification Method

Resumo

Many specific detection assays have been developed in order to efficiently detect GMOs (genetically modified organisms) in feed and food. Existing approaches to screen or detect GM crops always rely on thermal cycling device. Therefore, these rapid visual detection assays were developed and characterized for the four transgenic soybean (Glycine max (L.) Merr.) events MON87701, MON87705, MON87708, and MON87769 using loop-mediated isothermal amplification (LAMP) method. Four sets of specific primers were successfully designed to recognize the junction sequences of specific soybean events. The optimum primers and reaction temperature of each assay were tested respectively. Different kinds of genomic DNAs of transgenic events and non-transgenic crop samples were examined with the methods that indicated the good specificity of GM soybean species. The detection limit of each LAMP assays could reach 0.05%, showing sensitivity 10 times higher than that of the regular PCR method. Due to their simplicity, rapidness and low cost, the established LAMP assays would be practical for detecting the GM soybeans MON87701, MON87705, MON87708, MON87769 and their derivatives.