Comparative analysis of the effectiveness of STR and SNP markers for intraspecific and interspecific differentiation of the genus Ovis


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Abstract

A comparative study of the informativeness of SNP and STR markers for interspecific and intraspecific differentiation of the two species of the genus Ovis, snow sheep (O. nivicola) and domestic sheep (O. aries), was conducted. Eleven STR loci combined into two multiplex panels were examined. SNP analysis was performed with the DNA microarray OvineSNP50K BeadChip featuring 54241 SNPs. The possibility of clear differentiation of the studied Ovis species with both types of genetic markers was demonstrated. The advantages of SNP markers for intraspecific differentiation of the O. aries breeds and O. nivicola geographical groups were revealed. The areas of application of the studied types of DNA markers are discussed.

About the authors

T. E. Deniskova

Ernst All-Russia Research Institute for Animal Husbandry

Author for correspondence.
Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132

A. A. Sermyagin

Ernst All-Russia Research Institute for Animal Husbandry

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132

V. A. Bagirov

Ernst All-Russia Research Institute for Animal Husbandry

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132

I. M. Okhlopkov

Ernst All-Russia Research Institute for Animal Husbandry; Institute for Biological Problems of Cryolithozone

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132; Yakutsk, 677890

E. A. Gladyr

Ernst All-Russia Research Institute for Animal Husbandry

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132

R. V. Ivanov

Ernst All-Russia Research Institute for Animal Husbandry

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132

G. Brem

Ernst All-Russia Research Institute for Animal Husbandry; Institute of Animal Breeding and Genetics

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132; Vienna, A-1210

N. A. Zinovieva

Ernst All-Russia Research Institute for Animal Husbandry

Email: tandeniss@rambler.ru
Russian Federation, Dubrovitsy settlement, Moscow oblast, 142132

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