Email this article Widely Applicable PCR Markers for Sex Identification in Birds
- Authors: Romanov M.N.1, Betuel A.M.2, Chemnick L.G.3, Ryder O.A.3, Kulibaba R.O.4, Tereshchenko O.V.5, Payne W.S.6, Delekta P.C.6, Dodgson J.B.6, Tuttle E.M.2, Gonser R.A.2
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Affiliations:
- Comparative and Functional Genomics Group, School of Biosciences, University of Kent
- The Center for Genomic Advocacy, Department of Biology, Indiana State University
- Genetics Division, San Diego Zoo Institute for Conservation Research
- Institute of Animal Science, National Academy of Agrarian Sciences of Ukraine
- State Poultry Research Station, National Academy of Agrarian Sciences of Ukraine
- Department of Microbiology and Molecular Genetics, Biomedical and Physical Sciences Bldg., Michigan State University
- Issue: Vol 55, No 2 (2019)
- Pages: 220-231
- Section: Animal Genetics
- URL: https://journal-vniispk.ru/1022-7954/article/view/189240
- DOI: https://doi.org/10.1134/S1022795419020121
- ID: 189240
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Abstract
To aid in avian sex determination if birds are not sexually dimorphic and/or they are sexually immature, several molecular assays involving the polymerase chain reaction (PCR) have been developed. To test in a variety of domestic and wild avian species applicability of five sexing assays: previously described four assays based on nucleotide sequence differences between the Z and W copy of the chicken chromodomain-helicase-DNA-binding protein gene (CHD1Z and CHD1W), and a new sexing marker using the ubiquitin associated protein 2 (UBAP2) gene sequence. At least one molecular sexing marker was successful in 84 out of 88 examined species across 13 avian orders. These assays may be useful in breeding management of domestic and wild birds as well as in studies of avian ecology, population genetics, embryology and transgenesis.
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About the authors
M. N. Romanov
Comparative and Functional Genomics Group, School of Biosciences, University of Kent
Author for correspondence.
Email: m.romanov@kent.ac.uk
United Kingdom, Canterbury, Kent, CT2 7NJ
A. M. Betuel
The Center for Genomic Advocacy, Department of Biology, Indiana State University
Email: m.romanov@kent.ac.uk
United States, Terre Haute, IN 47809
L. G. Chemnick
Genetics Division, San Diego Zoo Institute for Conservation Research
Email: m.romanov@kent.ac.uk
United States, Escondido, 15600CA 92027
O. A. Ryder
Genetics Division, San Diego Zoo Institute for Conservation Research
Email: m.romanov@kent.ac.uk
United States, Escondido, 15600CA 92027
R. O. Kulibaba
Institute of Animal Science, National Academy of Agrarian Sciences of Ukraine
Email: m.romanov@kent.ac.uk
Ukraine, Kulinichi, Kharkov District, 62404
O. V. Tereshchenko
State Poultry Research Station, National Academy of Agrarian Sciences of Ukraine
Email: m.romanov@kent.ac.uk
Ukraine, Birky, Kharkiv region, 63421
W. S. Payne
Department of Microbiology and Molecular Genetics, Biomedical and Physical Sciences Bldg.,Michigan State University
Email: m.romanov@kent.ac.uk
United States, East Lansing, MI 48824
Ph. C. Delekta
Department of Microbiology and Molecular Genetics, Biomedical and Physical Sciences Bldg.,Michigan State University
Email: m.romanov@kent.ac.uk
United States, East Lansing, MI 48824
J. B. Dodgson
Department of Microbiology and Molecular Genetics, Biomedical and Physical Sciences Bldg.,Michigan State University
Email: m.romanov@kent.ac.uk
United States, East Lansing, MI 48824
E. M. Tuttle
The Center for Genomic Advocacy, Department of Biology, Indiana State University
Email: m.romanov@kent.ac.uk
United States, Terre Haute, IN 47809
R. A. Gonser
The Center for Genomic Advocacy, Department of Biology, Indiana State University
Email: m.romanov@kent.ac.uk
United States, Terre Haute, IN 47809
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