Email this article Establishing a Cultivable Cell Line of the Tick Dermacentor marginatus
- Authors: Rimikhanov N.I.1, Epova E.Y.2, Belyakova A.V.2, Lebedeva A.A.3, Mutnykh E.S.3, Biryukova Y.K.4, Zylkova M.V.4, Shibaeva A.V.2, Trubnikova E.V.4, Karataeva D.A.5, Akbaev R.M.5, Tyno Y.Y.5, Laypanov B.K.5
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Affiliations:
- Department of Veterinary Medicine, Moscow State University of Food Production
- Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
- Vavilov Institute of General Genetics, Russian Academy of Sciences
- Research Laboratory Genetika, Kursk State University
- Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
- Issue: Vol 55, No 8 (2019)
- Pages: 933-938
- Section: General Genetics
- URL: https://journal-vniispk.ru/1022-7954/article/view/189483
- DOI: https://doi.org/10.1134/S102279541908012X
- ID: 189483
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Abstract
Owing to the changes in the general ecological situation in Russia, livestock losses from ovine anaplasmosis have rapidly increased in recent years. The development of a vaccine against this disease is therefore all the more relevant. A continuous culture of tick cells could be used as an appropriate substrate for producing biomass of the pathogen of ovine anaplasmosis, Anaplasma ovis, for the purpose of manufacturing a cell-cultural inactivated vaccine. Such a continuous cell line of the tick Dermacentor marginatus, the definitive reservoir host of A. ovis, has been obtained by cultivation of homogenized tick eggs on a modified Leibovitz medium L15. The established cell line DM-77 has an undifferentiated phenotype: the cells are rounded, with large nuclei and well-visible nucleoli. By the end of the study, the cell line was passaged eight times and did not express signs of growth rate decrease. The duplication time was 2.8 ± 0.3 days for cultivation in a medium with 10% fetal serum at 32°C.
About the authors
N. I. Rimikhanov
Department of Veterinary Medicine, Moscow State University of Food Production
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 125080
E. Yu. Epova
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 119334
A. V. Belyakova
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 119334
A. A. Lebedeva
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 11991
E. S. Mutnykh
Vavilov Institute of General Genetics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 11991
Yu. K. Biryukova
Research Laboratory Genetika, Kursk State University
Author for correspondence.
Email: biriukova-ula@mail.ru
Russian Federation, Kursk, 305000
M. V. Zylkova
Research Laboratory Genetika, Kursk State University
Email: biriukova-ula@mail.ru
Russian Federation, Kursk, 305000
A. V. Shibaeva
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 119334
E. V. Trubnikova
Research Laboratory Genetika, Kursk State University
Email: biriukova-ula@mail.ru
Russian Federation, Kursk, 305000
D. A. Karataeva
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 109472
R. M. Akbaev
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 109472
Y. Y. Tyno
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 109472
B. K. Laypanov
Department of Parasitology and Veterinary-Sanitary Examination, Skryabin Moscow State Academyof Veterinary Medicine and Biotechnology
Email: biriukova-ula@mail.ru
Russian Federation, Moscow, 109472
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