Email this article A novel electrochemical biosensor for detection of cholesterol
- Authors: Xu L.1,2, Hou Y.3, Zhang M.4, Yang X.4, Jenkins G.3, Huang W.4, Yao C.1, Wu Q.4
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Affiliations:
- State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science
- Nan Jing College of Chemical Technology
- Key Laboratory for Organic Electronics & Information Displays (KLOEID)
- Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM)
- Issue: Vol 52, No 3 (2016)
- Pages: 239-244
- Section: Article
- URL: https://journal-vniispk.ru/1023-1935/article/view/187755
- DOI: https://doi.org/10.1134/S1023193516030101
- ID: 187755
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Abstract
We report on a highly sensitive electrochemical biosensor for determination of cholesterol. The biosensor was fabricated by co-immobilizing bi-enzymes, cholesterol oxidase (ChOx), and horseradish peroxidase (HRP). Voltammetric technique such as cyclic voltammetry and impedance experiment were used to study the characterization of modified electrode step by step. The developed sensor is cheap, disposable, portable and exhibits higher sensitivity. The biosensor expressed a wide linear range up to 300 mg dL–1 in a physiological condition (pH 7.0), with a correlation coefficient of 0.9969. A sensitivity of 13.28 μA mg–1 dL cm−2 which makes it very promising for the clinical determination of cholesterol.
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About the authors
Lin Xu
State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science; Nan Jing College of Chemical Technology
Email: iamqwu@njtech.edu.cn
China, 30 South Puzhu Road, Nanjing, 211816; 625 GeGuan Road, Nanjing, 210048
Yiting Hou
Key Laboratory for Organic Electronics & Information Displays (KLOEID)
Email: iamqwu@njtech.edu.cn
China, 9 Wenyuan Road, Yadong, Xincheng District, Nanjing, 210046
Mengdan Zhang
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM)
Email: iamqwu@njtech.edu.cn
China, 30 South Puzhu Road, Nanjing, 211816
Xin Yang
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM)
Email: iamqwu@njtech.edu.cn
China, 30 South Puzhu Road, Nanjing, 211816
Greath Jenkins
Key Laboratory for Organic Electronics & Information Displays (KLOEID)
Email: iamqwu@njtech.edu.cn
China, 9 Wenyuan Road, Yadong, Xincheng District, Nanjing, 210046
Wei Huang
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM)
Email: iamqwu@njtech.edu.cn
China, 30 South Puzhu Road, Nanjing, 211816
Cheng Yao
State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science
Author for correspondence.
Email: yaocheng@njtech.edu.cn
China, 30 South Puzhu Road, Nanjing, 211816
Qiong Wu
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM)
Author for correspondence.
Email: iamqwu@njtech.edu.cn
China, 30 South Puzhu Road, Nanjing, 211816
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