Protein-Based Nanobiosensor for Electrochemical Determination of Hydrogen Peroxide

Abstract

We designed a hydrogen peroxide biosensor. The electrochemical detection of H₂O₂ was performed based on immobilization of cobalt nanoparticles–ferritin (CoNPs–Fer) onto multiwalled carbon nanotubes (MWCNTS) ensnared into chitosan (CS) matrices. Electrochemical techniques such as differential pulse voltammetry (DPV) and cyclic voltammetry (CV) was used to check the property of biosensor. Energy-dispersive X-ray spectroscopy (EDXS) and field emission scanning electron microscopy (FESEM) techniques showed the prosperous immobilization of CoNPs–Fer on the modified GC electrode surface. The hydrogen peroxide-designed biosensor displayed a linear range from 0.2 to 14 nM (R² = 0.99), a detection limit of 1.29 nM (S/N = 3) and sensitivity of –0.1105 μA/nM.The apparent heterogeneous electron transfer rate constant (K_s) and the charge transfer coefficient (α) were gained 4.19 s^–1 and 0.49, respectively. This biosensor can detect hydrogen peroxide with high sensitivity, selectivity, and low detection limit.