Estimated Gene Expression of SNARE Proteins in Hyppocampus of Rats after Modelled Spaceflight

A. A. Perevezentsev; Перевезенцев А. А.; K. B. Lebedeva-Georgievskaya; Лебедева-Георгиевская К. Б.; O. S. Kuznetsova; Кузнецова О. С.; A. S. Shtemberg; Штемберг А. С.

doi:10.31857/S1027813323020103

Estimated Gene Expression of SNARE Proteins in Hyppocampus of Rats after Modelled Spaceflight

Authors: Perevezentsev A.A.¹, Lebedeva-Georgievskaya K.B.¹, Kuznetsova O.S.¹, Shtemberg A.S.¹
Affiliations:
1. Institute of BioMedical Problems of Russian Academy of Sciences
Issue: Vol 40, No 2 (2023)
Pages: 186-192
Section: Experimental Articles
URL: https://journal-vniispk.ru/1027-8133/article/view/142209
DOI: https://doi.org/10.31857/S1027813323020103
EDN: https://elibrary.ru/UCRDZM
ID: 142209

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Abstract
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Abstract

Our recent researches demonstrate shifts in brain activity of rats, both at behavioral and biochemical levels, induced by one of modeled impacts of interplanetary spaceflight, i.e. space radiation. Thus emerges a question, whether such shifts occur in molecular underlying processes of brain function. We have investigated the gene expression for proteins of SNARE (soluble NSF attachment receptor), responsible for fusion of transport vesicles with terminal membranes. Our result is an evidence of changes in some SNARE elements after irradiation and an indirect evidence of a link between nature of such changes and animal nervous system typology. Thus, in order, impairments of brain activity after model irradiation compared to such of an interplanetary flight are observed at every level of nervous system structure.

Keywords

ionizing radiation, cosmic rays, neurochemical, expression, SNARE, rats

References

Штемберг А.С., Лебедева-Георгиевская К.Б., Матвеева М.И., Кудрин В.С., Наркевич В.Б., Клодт П.М., Базян А.С. // Известия РАН. Серия Биологическая. 2014. Т. 2. С. 168–75.
Kokhan V.S., Lebedeva–Georgievskaya K.B., Kudrin V.S., Bazyan A.S., Maltsev A.V., Shtemberg A.S. // Life Sciences in Space Research. 2019. V. 20. P. 12–19.
Huang Ting-Ting. // Free Radical Research. 2012. V. 46. № 8. P. 951–958.
Штемберг А.С., Перевезенцев А.А., Лебедева-Георгиевская К.Б., Митрофанова О.В., Кудрин В.С., Базян А.С. // Радиационная биология. Радиоэкология. 2020. Т. 60. № 1. С. 51–62.
Shtemberg A.S., Lebedeva-Georgievskaya K.B., Matveeva M.I., Kudrin V.S. // Izvestiia Akademii Nauk. Seriia Biologicheskaia. 2014. № 2. P. 168–175.
Belyaeva A.G., Kudrin V.S., Koshlan I.V., Koshlan N.A., Isakova M.D., Bogdanova Y.V., Timoshenko G.N., Krasavin E.A., Blokhina T.M., Yashkina E.I., Osipov A.N., Nosovsky A.M., Perevezentsev A.A., Shtemberg A.S. // Life Sciences in Space Research. 2021. V. 30. P. 45–54.
Shi L., Adams M.M., Long A., Carter C.C., Bennett C., Sonntag W.E., Nicolle M.M., Robbins M., D’Agostino R., Brunso-Bechtold J.K. // Radiat. Res. 2006 V. 166. № 6. P. 892–899.
Poulose S.M., Bielinski D.F., Carrihill-Knoll K., Rabin B.M., Shukitt-Hale B. // Radiat. Res. 2011. V. 176. № 6. P. 761–769.
Berchtold N.C., Coleman P.D., Cribbs D.H., Rogers J., Gillen D.L., Cotman C.W. // Neurobiol. Aging. 2013. V. 34. № 6. P. 1653–1661.
Furong Cheng, Giorgio Vivacqua, Shun Yu // Journal of Chemical Neuroanatomy. 2011. V. 42. № 4. P. 242–248.
Julie Lotharius, Patrik Brundin. // Human Molecular Genetics. 2002. V. 11 № 20. P. 2395–2407.
Штемберг А.С., Перевезенцев А.А., Лебедева-Георгиевская К.Б., Митрофанова О.В., Кудрин В.С., Базян А.С. // Радиационная биология. Радиоэкология. 2020. Т. 60. № 1. С. 51–62.
Schiavo G., Stenbeck G., Rothman J.E., Söllner T.H. // Proceedings of the National Academy of Sciences of USA. 1997. V. 94. № 3. P. 997–1001.
Zhiping P. Pang, Ernestina Melicoff, Daniel Padgett, Yun Liu, Andrew F. Teich, Burton F. Dickey, Weichun Lin, Roberto Adachi, Thomas C. Südhof // The Journal of Neuroscience: The Official Journal of the Society for Neuroscience. 2006. V. 26. № 52. P. 13493–13504.
Pevsner J., Hsu S.C., Braun J.E., Calakos N., Ting A.E., Bennett M.K., Scheller R.H. // Neuron. 1994. V. 13. № 2. P. 353–361.
Robert D., Burgoyne, Jeff W. Barclay, Leo F. Ciufo, Margaret E. Graham, Mark T.W. Handley, Alan Morgan // Annals of the New York Academy of Sciences. 2009. V. 1152. P. 76–86.
Shyam S. Krishnakumar, Daniel T. Radoff, Daniel Kümmel, Claudio G. Giraudo, Feng Li, Lavan Khandan, Stephanie Wood Baguley, Jeff Coleman, Karin M. Reinisch, Frederic Pincet, James E. Rothman// Nature Structural & Molecular Biology. 2011. V. 18. № 8. P. 934–940.
Kuang Hu, Joe Carroll, Colin Rickman, Bazbek Davletov // The Journal of Biological Chemistry. 2002. V. 277. № 44. P. 41652–41656.
Симонов П.В. // Нейрофизиологический подход к анализу внутривидового поведения. М.: Наука, 1976. 160 с.
Micheli L., Creanza T.M., Ceccarelli M. et al. // Front. Cell. Dev. Biol. 2021. V. 9. № 696684.
Livak K.J., Schmittgen T.D. // Methods. 2001. V. 25. № 4. P. 402–408.