THE ROLE OF ADENOSINTRIPOSPHATE IN THE REGULATION OF HUMAN PERIPHERAL BLOOD NEUTROPHILES AND MONOCYTES DEGRANULATION IN VITRO
- Authors: Asadullina I.A.1, Serebryakova M.K.1, Kudryavtsev I.V.1,2
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Affiliations:
- Institute of Experimental Medicine
- N. N. Petrov Research Institute of Oncology
- Issue: Vol 20, No 2 (2017)
- Pages: 96-99
- Section: Articles
- URL: https://journal-vniispk.ru/1028-7221/article/view/119520
- ID: 119520
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Full Text
Abstract
At present, the metabolism of nucleotides is of great interest in the context of the regulation of functions of immunocompetent cells in inflammation. It is adenosine triphosphate that is attributed to many effects that cause chemotaxis, activation, degranulation of various subpopulations of leukocytes. In this study, the effect of ATP on the degranulation of neutrophils and various subpopulations of human peripheral blood monocytes in vitro was studied using flow cytometry. We have shown that the addition of ATP at concentrations of 1 mM and 0.1 mM caused a significant increase in the expression level of the СD66b degranulation marker on neutrophils. An increase in the expression level of the СD63 degranulation marker on a subpopulation of "classical" monocytes expressing СD14 is shown only at the time of 15 min of incubation, while subpopulations of "nonclassical" monocytes expressing СD16 did not respond to the application of this stimulant.
About the authors
I. A. Asadullina
Institute of Experimental Medicine
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
M. K. Serebryakova
Institute of Experimental Medicine
Email: noemail@neicon.ru
Russian Federation
I. V. Kudryavtsev
Institute of Experimental Medicine; N. N. Petrov Research Institute of Oncology
Email: noemail@neicon.ru
Russian Federation
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