IDIOTYPIC AND ANTI-IDIOTYPIC ANTIBODIES AGAINST POLYCYCLIC AROMATIC HYDROCARBONS IN HUMAN BLOOD SERUM ARE NEW BIOMARKERS OF LUNG CANCER
- Authors: Grebenshchikov I.S.1, Studennikov A.E.1, Glushkov A.N.1, Ustinov V.A.1
-
Affiliations:
- Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Institute of Human Ecology
- Issue: Vol 22, No 2-1 (2019)
- Pages: 221-223
- Section: ORIGINAL ARTICLES
- URL: https://journal-vniispk.ru/1028-7221/article/view/119611
- DOI: https://doi.org/10.31857/S102872210006581-4
- ID: 119611
Cite item
Full Text
Abstract
We studied the levels of idiotypic and anti-idiotypic antibodies against benzo[a]pyrene in the blood serum of lung cancer patients and healthy donors by direct ELISA. It was shown that antibody levels between these groups was statistically signifi cantly different. Based on these results, a quantify antibodies immunoassay in the human serum was developed using a multiplex method.
About the authors
I. S. Grebenshchikov
Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Institute of Human Ecology
Author for correspondence.
Email: GrebenshchikovIS@ihe.sbras.ru
Graduate Student, Leading Technologist in the Laboratory of Biotechnology,
Kemerovo
Russian FederationA. E. Studennikov
Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Institute of Human Ecology
Email: fake@neicon.ru
Leading Technologist in the Laboratory of Biotechnology,
Kemerovo
Russian FederationA. N. Glushkov
Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Institute of Human Ecology
Email: fake@neicon.ru
MD, Chef of the Institute of Human Ecology,
Kemerovo
Russian FederationV. A. Ustinov
Federal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Institute of Human Ecology
Email: fake@neicon.ru
PhD, Leader the Laboratory of Biotechnology,
Kemerovo
Russian FederationReferences
- Kim K. H., Jahan S. A., Kabir E., Brown R. J. A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health eff ects. Environ Int. 2013, 60, 71–80.
- Ustinov V.A., Matveeva V.A., Kostyanko M.A., Glushkov A.N. Antibodies against benzo[a]pyrene in immunized mouse and in lung cancer patients. Exp Oncol. 2013, 35, 207–210.
- Buratti M., Campo L., Fustinoni S., Cirla P.E., Martinotti I., Cavallo D., Foa V. Urinary hydroxylated metabolites of polycyclic aromatic hydrocarbons as biomarkers of exposure in asphalt workers. Biomarkers. 2007, 12(3), 221–239.
- Zheng W, He L. Multiplexed Immunoassays. Advanced Techniques in Immunoassays published by Academic Pages, 5 Penn Plaza, 19th Floor, New York, NY10001, USA, 2017, 143–164.
Supplementary files
