REACTION OF ACTIVATED MONONUCLEATED CELLS TO CO-CULTIVATION WITH CALCIUM PHOSPHATE COATING OF TITANIUM MATRIX

L. S. Litvinova; Литвинова Л. С.; K. A. Yurova; Юрова К. А.; V. V. Shupletsova; Шуплецова В. В.; O. G. Khaziakhmatova; Хазиахматова О. Г.; E. S. Melashchenko; Мелащенко Е. С.; V. V. Malashchenko; Малащенко В. В.; E. O. Shunkin; Шунькин Е. О.; Yu. P. Sharkeev; Шаркеев Ю. П.; E. G. Komarova; Комарова Е. Г.; M. B. Sedelnikova; Седельникова М. Б.; I. K. Norkin; Норкин И. К.; K. I. Prokin; Прокин К. И.; P. A. Ivanov; Иванов П. А.; I. A. Khlusov; Хлусов И. А.

doi:10.31857/S102872210007070-2

REACTION OF ACTIVATED MONONUCLEATED CELLS TO CO-CULTIVATION WITH CALCIUM PHOSPHATE COATING OF TITANIUM MATRIX

Authors: Litvinova L.S.¹, Yurova K.A.¹, Shupletsova V.V.¹, Khaziakhmatova O.G.¹, Melashchenko E.S.¹, Malashchenko V.V.¹, Shunkin E.O.¹, Sharkeev Y.P.², Komarova E.G.², Sedelnikova M.B.², Norkin I.K.¹, Prokin K.I.¹, Ivanov P.A.¹, Khlusov I.A.³^,2
Affiliations:
1. Immanuel Kant Baltic Federal University
2. Tomsk Polytechnic University
3. Siberian State Medical University
Issue: Vol 22, No 4 (2019)
Pages: 1497-1499
Section: ORIGINAL ARTICLES
URL: https://journal-vniispk.ru/1028-7221/article/view/119617
DOI: https://doi.org/10.31857/S102872210007070-2
ID: 119617

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Abstract

The reactions of mononuclear blood cells (MNC) were evaluated under cocultivation with titanium substrates carrying a bilateral calcium phosphate coating (CP) under conditions of additional activation of the T-cell receptor. It has been established that in the activation 3D model, in contact with CP-coating, the secretion of pro- and anti-inflammatory cytokines (IL-2, TNF-α, IL-10, IL-4) increases, against the background of a decrease in the viability of the MNC culture and changes in their immunophenotype, in the direction of increasing the number of cells expressing CD95+ and CD45R0+ (the number of CD25+ cells has not changed), which may indicate that the MNC is hyperactive in the 3D model when it is stimulated by an activator of T cells.