EFFECT OF ERYTHROPOIETIN ON FUNCTIONAL ACTIVITY OF BLOOD NEUTROPHILS OF PREMATURE INFANTS IN EXPERIMENT INVITRO
- Authors: Osikov M.V.1, Almukhametova O.N.1, Vorgova L.V.1
-
Affiliations:
- South Ural State Medical University, Russian Ministry of Health
- Issue: Vol 20, No 2 (2017)
- Pages: 182-185
- Section: Articles
- URL: https://journal-vniispk.ru/1028-7221/article/view/119635
- ID: 119635
Cite item
Full Text
Abstract
Aim of the study - to investigate the effect of erythropoietin (EPO) on the absorption and NBT-re-ducing capacity of neutrophils isolated from the blood of preterm neonates (PN) in vitro. The study was performed on 18 infants: PN from premature birth (PB) (n=6); PN from early preterm birth (EPB) (n=6); PN from a very early preterm birth (VEPB) (n=6). The EPO used in concentrations of 30 IU/l; 15 IU/l; 7,5 IU/l; 3,75 IU/L. Evaluated the absorption capacity and oxygen-dependent metabolism of neutrophils isolated from umbilical cord blood. Have PN from EPB and VEPB, EPO in in vitro experimental conditions increases, and PN from PB - reduces the absorption capacity and NBT-reducing capacity of blood neutrophils. EPO effect on the absorption capacity of blood neutrophils in in vitro experimental conditions does not depend on gestational age and manifests itself in small doses (3,75 IU/l and 7.5 IU/l), and NBT-reducing capacity of blood neutrophils in large doses (15 IU/l and 30 IU/l).
About the authors
M. V. Osikov
South Ural State Medical University, Russian Ministry of Health
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
O. N. Almukhametova
South Ural State Medical University, Russian Ministry of Health
Email: noemail@neicon.ru
Russian Federation
L. V. Vorgova
South Ural State Medical University, Russian Ministry of Health
Email: noemail@neicon.ru
Russian Federation
References
- Осиков М. В., Ахматов В. Ю., Телешева Л. Ф., Федосов А. А., Агеев Ю. И. и др. Плейотропные эффекты эритропоэтина при хронической почечной недостаточности. Фундаментальные исследования 2013, 7-1, 218-224.
- Осиков М. В., Телешева Л. Ф., Кишкин А. М., Федосов А. А. Влияние эритропоэтина на показатели врожденного иммунитета в ранние сроки экспериментальной термической травмы. Фундаментальные исследования 2014, 7-1, 334-339.
- Осиков М. В., Ахматов К. В., Кривохижина Л. В. Патофизиологический анализ влияния эритро-поэтина на психологический статус у больных хронической почечной недостаточностью, находящихся на гемодиализе. Человек. Спорт. Медицина 2010, 19(195), 110-116.
- Logan J. W., Allred E. N., Fichorova R. N., Engelke S., Dammann O. et al. Endogenous erythropoietin varies significantly with inflammation-related proteins in extremely premature newborns. Cytokine 2014, 69(1), 22-28.
- Ohls R. K., Kamath-Rayne B.D., Christensen R. D., Wiedmeier S. E., Rosenberg A. et al. Cognitive outcomes of preterm infants randomized to darbepoetin, erythropoietin, or placebo. Pediatrics 2014, 133(6), 1023-1030.
Supplementary files
