CHARACTERIZATION OF ANTIMICROBIAL PEPTIDES FROM DOMESTIC HORSE'S PLATELETS

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Abstract

The aim of this work was to investigate an antimicrobial properties of platelets of domestic horse (Equus ferus caballus) and isolate an antimicrobial components. Using a liquid chromatography technique (RP-HPLC) ten fractions were collected from platelet's acid extract. Among these a four fractions demonstrated antimicrobial activity against S.aureus.

About the authors

Yu. I. Peshkova

Orenburg State Agrarian University

Author for correspondence.
Russian Federation

M. V.1· Sycheva

Orenburg State Agrarian University; Institute of Cellular and Intracellular Symbiosis UrD RAS

Russian Federation

E. A. Rogozhin

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of bioorganic chemistry RAS

Russian Federation

A. S. Vasylchenko

Orenburg State Agrarian University

Russian Federation

O. L. Kartashova

Institute of Cellular and Intracellular Symbiosis UrD RAS

Russian Federation

References

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  2. Micota B., Sadowska B., RYialska B. The role of blood platelets in infections. Postepy Hig. Med. Dosw. (Online). 2015, 69, 624-632.
  3. Aktan I., Dunkel B., Cunningham F. M. Equine platelets inhibit E. coli growth and can be activated by bacterial lipopolysaccharide and lipoteichoic acid although superoxide anion production does not occur and platelet activation is not associated with enhanced production by neutrophils. Vet. Immunol. Immunopathol. 2013, 152(3-4), 209-217.
  4. Сычева М. В., Васильченко А. С., Рогожин Е. А., Пашкова Т. М., Попова Л. П. и др. Биологическая активность антимикробных пептидов из тромбоцитов кур. Журнал микробиологии, эпидемиологии и иммунобиологии. 2016, 2, 24-30.
  5. Wiegand I., Hilpert K., Hancock R. E.W. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols. 2008, 3(2), 163-175.

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Copyright (c) 2017 Peshkova Y.I., Sycheva M.V., Rogozhin E.A., Vasylchenko A.S., Kartashova O.L.

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