Elisa detection of cytokine levels to determine the dynamics of the flow inflammatory process
- Authors: Aznabaeva L..1, Zherebyatyeva О..1, Mikhailova E..1, Kirgozova S..1, Machalova G..1, Fomina M..1, Elagina N..1, Fedorova T..1, Mironov A..2
-
Affiliations:
- Orenburg State Medical University
- The G.N. Gabrichevskii Moscow research institute of epidemiology and microbiology of Rospotrebnadzor of Russia
- Issue: Vol 21, No 3 (2018)
- Pages: 195-198
- Section: ORIGINAL ARTICLES
- URL: https://journal-vniispk.ru/1028-7221/article/view/119832
- DOI: https://doi.org/10.31857/S102872210002380-3
- ID: 119832
Cite item
Full Text
Abstract
The work carried out an assessment using ELISA level of serum cytokines and biological fl uids of persons who have undergone purulent-infl ammatory diseases of the urogenital tract. The object of the study was the cytokine profi le of 98 men with gonococcal infection and 40 patients with nonspecifi c urethritis. The study showed that the established values of cytokine levels were used as markers of the volume of urogenital infl ammation, which served as the basis for the development of a method of diagnosis and prediction of the course of the infectious process. The revealed diff erences in the immunocytocin profi le can contribute to the disclosure of the mechanisms of formation of various forms of infection, as well as to determine the methodological and tactical approaches to the treatment and rehabilitation of patients with sexual and reproductive health disorders.
About the authors
L. . Aznabaeva
Orenburg State Medical University
Author for correspondence.
Russian Federation
О. . Zherebyatyeva
Orenburg State Medical UniversityRussian Federation
E. . Mikhailova
Orenburg State Medical UniversityRussian Federation
S. . Kirgozova
Orenburg State Medical UniversityRussian Federation
G. . Machalova
Orenburg State Medical UniversityRussian Federation
M. . Fomina
Orenburg State Medical UniversityRussian Federation
N. . Elagina
Orenburg State Medical UniversityRussian Federation
T. . Fedorova
Orenburg State Medical UniversityRussian Federation
A. . Mironov
The G.N. Gabrichevskii Moscow research institute of epidemiology and microbiology of Rospotrebnadzor of RussiaRussian Federation
References
- Gan S. D., Patel K. R. Enzyme immunoassay and enzymelinked immunosorbent assay. J. Invest Dermatol, 2013, 9, Vol. 133 (9), 12.
- Белая О. Ф., Пак С. Г. Пути совершенствования лабораторной диагностики инфекционных заболеваний. Вестник РАМН, 2010, 11, 50–53.
- Долгов В. В., Ракова Н. Г., Колупаев В. Е., Рытикова Н. С. Иммуноферментный анализ в кли нико-ди агностических лабораториях. М. – Тверь: Триада. 2007. 320 с.
- Самойленков П. В. Принципы иммуноферментного анализа, основные виды ИФА, применение в диагностике. Москва, 2009, РГМУ.
- Baggiolini M., Dewald B., Moser B. Human chemokines: an update. Annu. Rev. Immunol., 1997, Vol. 15, 675–705.
- Dinarello C. The biological properties of interleukin-1. Eur. Cytokine Netw., 1994, Vol. 5, 517–526.
- Курынин Р. М. Достижения отечетственной молекулярной генетики для клинической онкоурологии. Урология сегодня, 2009, 4, 17.
- Герасимов А. Н. Медицинская статистика. М. МИА. 2007.
- Жеребятьева О. О., Сетко Н. П., Михайлова Е. А., Киргизова С. Б., Константинова О. Д., Кшнясева С. К., Махалова Г. О., Ляшенко И. Э. Иммунологические критерии прогнозирования вторичного бесплодия. Российский иммунологический журнал 2015, 9(18), 2 (1), 378–379.
- Демьянов А. В., Котов А. Ю., Симбирцев А. С. Диагностическая ценность исследования уровней цитокинов в клинической практике. Цитокины и воспаление, 2003, Т. 2, 3, 20–35.
Supplementary files
