Assessment of lymphocyte populations and their subsets in the patients with acute coronary syndrome
- Authors: Safronova E.A.1, Ryabova L.V.1
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Affiliations:
- South Ural State Medical University
- Issue: Vol 25, No 3 (2022)
- Pages: 313-320
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/1028-7221/article/view/120208
- DOI: https://doi.org/10.46235/1028-7221-1135-AOL
- ID: 120208
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Abstract
We examined 23 patients aged 40 to 65 years (mean age 54.52±6.72) with a diagnosis of acute coronary syndrome (ACS) at admission, who underwent emergency or delayed coronary stenting a day later. All patients had arterial hypertension as a concomitant disease. Upon additional examination, blood troponin levels were determined, ECG was performed in the time dynamics. Acute myocardial infarction with ST elevation was diagnosed in 7 patients, infarction without ST elevation, in 6 patients, the unstable angina rest, in the rest of this group (Grace risk from 75 to 150 points, on average, 107.7±27.16 points). To assess the immune status, especially, lymphocyte populations and subsets we used standardized techniques, i.e., flow cytometric assays with Navios cytofluorimeter (Beckman Coulter, USA). The following subpopulations were determined: CD45+ (panleukocyte marker for gating lymphocytes), CD45+, CD3+ (T cells), CD45+, CD3+, CD4+ (helper inducers), CD45+, CD3+, CD8+ (cytotoxic T cells), CD45+, CD3+CD16+, CD56+ (TNK cells) CD45+, CD3-, CD16+, CD56+ (natural killer cells), CD45+, CD3-, CD19+CD5+ (B cells), CD45+, CD3+, CD4+, CD25+ (activated helpers, early activation phase), CD45+, CD3+, HLA-DR (activated T lymphocytes – late activation phase). The data obtained indicate that the relative indices of T helper subpopulations, T cells at early and late activation step, and B lymphocytes were increased in the patients with acute coronary syndrome, compared with control group. At the same time, there is a trend for increasing absolute values of these indexes. The subpopulation of TNK lymphocytes proved to be significantly increased both in relative and absolute values, whereas percentages of CD45+CD3+CD19- (p < 0.01) and T cytotoxic lymphocytes (p < 0.001) were decreased and showed the same trend in absolute terms. The ratio of CD4/CD8 lymphocytes was almost doubled (p < 0.001), due to increased content of T-helpers and decrease in cytotoxic T lymphocytes. In clinical blood analyses of ACS patients a tendency for leukocytosis was shown, (10.15±5.22), with a shift to the band forms.
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##article.viewOnOriginalSite##About the authors
E. A. Safronova
South Ural State Medical University
Author for correspondence.
Email: safronovaeleonora68@gmail.com
PhD (Medicine), Associate Professor, Department of Polyclinic Therapy and Clinical Pharmacology
Russian Federation, ChelyabinskL. V. Ryabova
South Ural State Medical University
Email: safronovaeleonora68@gmail.com
PhD, MD (Medicine), Professor, Department of Life Safety, Disaster Medicine, Critical and Emergency Medical Care
Russian Federation, ChelyabinskReferences
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