Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma

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Abstract

HLA-E is a minor understudied non-classical HLA genes. HLA-E transcription is revealed in many cell types, especially, in immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of HLA-E on CD8+, CD4+ and CD14+ cells in conditionallу healthy donors and in the patients with bronchial asthma (BA) and rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA patients (n = 15), BA (n = 11) and healthy donors were separated from peripheral blood in Ficoll-Urographin density gradient (1.077 g/mL). The cells were then stained with fluorochrome-conjugated monoclonal antibodies: anti-CD3-APC, anti-CD4-APC-Cy7, anti-CD-14-FITC, and anti-HLA-E-PerCP/Cy5. The cell phenotype was analyzed by flow cytometry with FACS Canto II (BD Biosciences, USA). We have found an increased expression of HLA-E on CD8+, CD4+Т cells, like as on CD14+ cells (monocytes) in the RA patients, when compared with BA patients. We have also shown significant differences of HLA-E expression on CD8+Т cells between the conditionally healthy donors and RA patients.

About the authors

Olga S. Boeva

Research Institute of Fundamental and Clinical Immunology; Novosibirsk State University

Author for correspondence.
Email: starchenkova97@gmail.com

Student, Novosibirsk State University; Assistant Researcher, Laboratory of Clinical Immunopathology, Research Institute of Fundamental and Clinical Immunology

Russian Federation, Novosibirsk; Novosibirsk

V. I. Borisevich

Novosibirsk State Medical University

Email: borvad2001@mail.ru

Student, Novosibirsk State Medical University

Russian Federation, Novosibirsk

V. A. Kozlov

Research Institute of Fundamental and Clinical Immunology

Email: vakoz40@yandex.ru

PhD, MD (Medicine), Professor, Full Member, Russian Academy of Sciences, Head, Laboratory of Clinical Immunopathology, Research Director, Research Institute of Fundamental and Clinical Immunology

Russian Federation, Novosibirsk

D. V. Vladimirovna

Research Institute of Fundamental and Clinical Immunology

Email: immunology@mail.ru

PhD (Medicine), Allergologist, Head, Department of Allergology, Research Institute of Fundamental and Clinical Immunology

Russian Federation, Novosibirsk

A. E. Sizikov

Research Institute of Fundamental and Clinical Immunology

Email: sizikovae@niikim.ru

PhD (Medicine), Rheumatologist, Head, Department of Rheumatology Research Institute of Fundamental and Clinical Immunology

Russian Federation, Novosibirsk

E. A. Pashkina

Research Institute of Fundamental and Clinical Immunology

Email: pashkina.e.a@yandex.ru

PhD (Biology), Senior Research Associate, Laboratory of Clinical Immunopathology, Research Institute of Fundamental and Clinical Immunology

Russian Federation, Novosibirsk

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2. Figure 1. Expression of HLA-E molecules on CD8+T cells in healthy donors and patients with bronchial asthma and rheumatoid arthritis

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3. Figure 2. Expression of HLA-E molecules on CD4+T cells in healthy donors and patients with bronchial asthma and rheumatoid arthritis

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4. Figure 3. Expression of HLA-E molecules on CD14+T cells in healthy donors and patients with bronchial asthma and rheumatoid arthritis

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Copyright (c) 2023 Boeva O.S., Borisevich V.I., Kozlov V.A., Vladimirovna D.V., Sizikov A.E., Pashkina E.A.

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