The expression patterns of the inhibitory receptors PD-1 and TIGIT on CD4+ and CD8+T lymphocytes at different stages of differentiation
- Authors: Vlasova V.V.1, Saidakova E.V.1
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Affiliations:
- Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences
- Issue: Vol 27, No 3 (2024)
- Pages: 553-558
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/1028-7221/article/view/267523
- DOI: https://doi.org/10.46235/1028-7221-16619-TEP
- ID: 267523
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Abstract
T lymphocytes are a highly diverse group of cells that play a pivotal role in the adaptive immune response. The T cell population consists of two subsets: CD4+T-helper cells and CD8+ cytotoxic T lymphocytes, each comprising cells with varying functionality and maturity levels. Inhibitory receptors such as PD-1 and TIGIT tightly regulate T lymphocyte functions to maintain immune homeostasis. However, the presence of inhibitory receptors on T cells is also associated with exhaustion. The specific characteristics of inhibitory receptor expression on CD4+ and CD8+T lymphocyte subsets are not fully understood. This study aimed to assess the expression of inhibitory receptors PD-1 and TIGIT on different subsets of CD4+ and CD8+T lymphocytes in healthy individuals. The study involved 10 relatively healthy volunteers, averaging 43 years. T lymphocytes subsets were identified using flow cytometry. CD4+ and CD8+T cells were classified as naive (CD45R0-CCR7+), central memory (CD45R0+CCR7+), effector memory (CD45R0+CCR7-), or terminally differentiated effectors (CD45R0-CCR7-) followed by analysis of PD-1 and TIGIT expression. The study showed that the expression of suppressor molecules PD-1 and TIGIT on T lymphocytes in healthy individuals is closely linked to their differentiation stage. The presence of cells carrying PD-1 and TIGIT receptors was significantly lower in naive T lymphocytes compared to more mature subsets (p < 0.05). Affiliation with CD4+ or CD8+T cells also significantly influenced the nature of inhibitory receptor expression. CD8+T lymphocytes had more TIGIT-positive elements than CD4+T cells (p < 0.01). Moreover, unlike PD-1, TIGIT was found on most memory and terminally differentiated effector CD8+T lymphocytes. These findings improve our understanding of how inhibitory receptors regulate T cell functions and emphasize the need to reconsider how we interpret data in the context of T lymphocyte exhaustion.
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##article.viewOnOriginalSite##About the authors
V. V. Vlasova
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences
Author for correspondence.
Email: violetbaudelaire73@gmail.com
Junior Research Associate, Laboratory of Molecular Immunology
Russian Federation, PermE. V. Saidakova
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: violetbaudelaire73@gmail.com
PhD, MD (Biology), Head, Laboratory of Molecular Immunology
Russian Federation, PermReferences
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