A view of immunologic male infertility from the perspective of preserving seminal fluid homeostasis
- Authors: Minasova A.A.1, Savochkina A.Y.1, Nokhrin D.Y.2, Sharabakina K.A.1, Pashkina N.V.3, Nikushkina K.V.1
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Affiliations:
- South-Ural State Medical University
- Chelyabinsk State University
- Medical Center “Lotos”
- Issue: Vol 27, No 4 (2024)
- Pages: 967-974
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/1028-7221/article/view/267914
- DOI: https://doi.org/10.46235/1028-7221-16904-AVO
- ID: 267914
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Abstract
The basis of the pathogenesis of immunologic male infertility is the production of antisperm antibodies against sperm antigens by plasmocytes. Extracellular nucleic acids, among other antigens, are perceived as DAMPs. The clearance system for biological fluids is complex. Endonucleases are responsible for the utilization of extracellular DNA. Among endonucleases, DNase 1 is the most studied. Its concentration in biological fluids is higher than others. For the first time, we determined the concentration of DNase 1 in the seminal fluid of men of reproductive age. DNase 1 concentration values were compared with the level of antisperm antibodies in the ejaculate, after which the relationship between the enzyme level and standard spermogram parameters was determined. The material for the study was the ejaculate of 44 relatively healthy men of reproductive age. During spermiological analysis, macroscopic and microscopic parameters of seminal fluid were determined, the concentration of deoxyribonuclease 1 (DNase 1) in the material was determined by enzyme immunoassay, and the presence of antisperm antibodies was assessed using a direct diagnostic method – the MAR test. Statistical processing of the obtained data was carried out using analytical software PAST v. 4.06. Reference intervals for DNase 1 concentrations were calculated using the MedCalc v. 17.4 program. The relationship between spermogram parameters and DNase 1 concentration was assessed by calculating Spearman’s linear rank correlation coefficients. As a result, a negative correlation was found between the DNase 1 ratio and such ejaculate parameters as liquefaction time (rS = -0.37; p = 0.013) and viscosity (rS = -0.37; p = 0.013), as well as the level of antisperm antibodies A (rS = -0.43; p = 0.003) and G (rS = -0.33; p = 0.027), the mucous component of the ejaculate (rS = -0.37; p = 0.012) and agglutination of male germ cells (rS = -0.33; p = 0.029). According to the data obtained, the optimal level of DNase 1 is directly related to the quality of the ejaculate. Determination of DNase 1 concentration can serve as an additional diagnostic criterion for male immunological infertility. These data will expand our understanding of a man’s fertile potential.
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##article.viewOnOriginalSite##About the authors
A. A. Minasova
South-Ural State Medical University
Author for correspondence.
Email: pandora_anna@mail.ru
PhD (Biology), Senior Research Associate, Research Institute of Immunology
Russian Federation, 64 Vorovsky St, Chelyabinsk, 454092A. Yu. Savochkina
South-Ural State Medical University
Email: pandora_anna@mail.ru
PhD, MD (Medicine), Associate Professor, Head, Department of Microbiology, Virology and Immunology
Russian Federation, 64 Vorovsky St, Chelyabinsk, 454092D. Yu. Nokhrin
Chelyabinsk State University
Email: pandora_anna@mail.ru
PhD (Biology), Associate Professor, Department of Microbiology, Immunology and General Biology
Russian Federation, ChelyabinskK. A. Sharabakina
South-Ural State Medical University
Email: pandora_anna@mail.ru
Senior Laboratory Assistant, Research Institute of Immunology
Russian Federation, 64 Vorovsky St, Chelyabinsk, 454092N. V. Pashkina
Medical Center “Lotos”
Email: pandora_anna@mail.ru
Obstetrician-Gynecologist, Reproductologist
Russian Federation, ChelyabinskK. V. Nikushkina
South-Ural State Medical University
Email: pandora_anna@mail.ru
PhD (Medicine), Leading Research Associate, Research Institute of Immunology
Russian Federation, 64 Vorovsky St, Chelyabinsk, 454092References
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