Indexes of innate immunity in patients with abdominal sepsis with different outcomes
- 作者: Osikov M.V.1,2, Konashov A.G.1,3, Boyko M.S.1, Gusev A.V.1,2, Konashov V.A.1,3
-
隶属关系:
- South Ural State Medical University
- Chelyabinsk Regional Clinical Hospital
- City Clinical Hospital No. 8
- 期: 卷 28, 编号 3 (2025)
- 页面: 673-680
- 栏目: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/1028-7221/article/view/319918
- DOI: https://doi.org/10.46235/1028-7221-17215-IOI
- ID: 319918
如何引用文章
全文:
详细
Innate immunity plays a critical role in early recognition and response to pathogens, and its study in abdominal sepsis (AS) is important for pathogenesis, early diagnosis, prognosis, and therapeutic strategy. The aim of our study was to search for indices of innate immunity in the dynamics of AS dependent on its clinical outcomes. The study was conducted in a group of 64 patients with AS aged 32-82 years, who were divided in two groups: (1) 46 patients with a favorable outcome, and (2) 18 cases with a fatal outcome. The studies were conducted on days 1, 3, and 7. The total number of leukocytes was examined using a Sysmex XT- 1800i/XT-2000i hematology analyzer (Japan); functional activity of neutrophils was measured by phagocytosis activity and intensity, and oxygen–dependent metabolism. The program “Statistica 10.0 for Windows” was used for statistical evaluation. It was found that, in AS patients, the number of metamyelocytes, rod-shaped, segmented neutrophils increases in the blood within 1-7 days of follow-up, regardless of clinical outcome; in cases of negative outcome of AS, neutrophilic leukocytosis is more pronounced, and the number of monocytes and eosinophils increases. In patients with AS, regardless of the outcome, the absorption capacity of blood neutrophils increases, along with increased oxygen-dependent metabolism in spontaneous mode, and its inhibition in induced mode. Inn cases of unfavorable outcome of AS, we observed elevation of absorption capacity of blood neutrophils on days 1-7 of follow-up, along with inhibition of oxygen-dependent neutrophil metabolism in spontaneous and induced modes, mainly, on days 3 and 7 of follow-up.
作者简介
M. Osikov
South Ural State Medical University; Chelyabinsk Regional Clinical Hospital
Email: ritkaboyko@yandex.ru
PhD, MD (Medicine), Professor, Head, Department of Pathophysiology, Head of the Department for Scientific and Innovative Work; Head, Department of Research
俄罗斯联邦, Chelyabinsk; ChelyabinskA. Konashov
South Ural State Medical University; City Clinical Hospital No. 8
Email: ritkaboyko@yandex.ru
PhD (Medicine), Associate Professor, Department of Pathophysiology; Deputy Chief Physician for the Medical Unit, Anesthesiologist-Intensive Care
俄罗斯联邦, Chelyabinsk; ChelyabinskMargarita Boyko
South Ural State Medical University
编辑信件的主要联系方式.
Email: ritkaboyko@yandex.ru
PhD (Medicine), Associate Professor, Department of Pathophysiology
俄罗斯联邦, ChelyabinskA. Gusev
South Ural State Medical University; Chelyabinsk Regional Clinical Hospital
Email: ritkaboyko@yandex.ru
Assistant Professor, Department of Pathophysiology; Anesthesiologist-Intensive Care
俄罗斯联邦, Chelyabinsk; ChelyabinskV. Konashov
South Ural State Medical University; City Clinical Hospital No. 8
Email: ritkaboyko@yandex.ru
Assistant Professor, Department of Pathophysiology; Trainee Physician, Intensive Care Unit
俄罗斯联邦, Chelyabinsk; Chelyabinsk参考
- Стрельцова Е.И., Пешкова И.В., Саматов И.Ю., Валеева В.А., Верещагин Е.И. Лимфопения как фактор, определяющий тяжесть сепсиса, как точный критерий диагностики и как объект терапии // Journal of Siberian Medical Sciences, 2020. № 3. С. 108-125. [Streltsova E.I., Peshkova I.V., Samatov I.Yu., Valeeva V.A., Vereshchagin E.I. Lymphopenia as a factor determining the severity of sepsis, as an accurate diagnostic criterion and as an object of therapy. Journal of Siberian Medical Sciences = Journal of Siberian Medical Sciences, 2020, no. 3, рр. 108-125.
- Фрейдлин И.С. Методы изучения фагоцитирующих клеток при оценке иммунного статуса человека: учебное пособие. Л., 1986. 37 с. [Freidlin I.S. Methods of studying phagocytic cells in the assessment of human immune status: textbook]. Leningrad, 1986. 37 p.
- Jarczak D., Nierhaus A. Cytokine storm-definition, causes, and implications. Int. J. Mol. Sci., 2022, Vol. 23, no. 19, 11740. doi: 10.3390/ijms231911740.
- Liu S., Li Y., She F., Zhao X., Yao Y. Predictive value of immune cell counts and neutrophil-to-lymphocyte ratio for 28-day mortality in patients with sepsis caused by intra-abdominal infection. Burns Trauma, 2021, Vol. 9, tkaa040. doi: 10.1093/burnst/tkaa040.
- Locati M., Curtale G., Mantovani A. Diversity, mechanisms, and significance of macrophage plasticity. Annu. Rev. Pathol., 2020, Vol. 15, pp. 123-147.
- Lu J., Liu J., Zhu L., Zhang Y., Li A. The effect of age on the clinical characteristics and innate immune cell function in the patients with abdominal sepsis. Front. Physiol., 2022, Vol. 13, 952434. doi: 10.3389/fphys.2022.952434.
- Nedeva C., Menassa J., Duan M., Liu C., Doerflinger M., Kueh A.J., Herold M.J., Fonseka P., Phan T.K., Faou P., Rajapaksha H., Chen W., Hulett M.D., Puthalakath H. TREML4 receptor regulates inflammation and innate immune cell death during polymicrobial sepsis. Nat. Immunol., 2020, Vol. 21, no. 12, pp. 1585-1596.
- Ramoni D., Tirandi A., Montecucco F., Liberale L. Sepsis in elderly patients: the role of neutrophils in pathophysiology and therapy. Intern. Emerg. Med., 2024, Vol. 19, no. 4, pp. 901-917.
- Scozzi D., Liao F., Krupnick A.S., Kreisel D., Gelman A.E. The role of neutrophil extracellular traps in acute lung injury. Front. Immunol., 2022, Vol. 13, 953195. doi: 10.3389/fimmu.2022.953195.
- Singer M., Deutschman C.S., Seymour C.W., Shankar-Hari M., Annane D., Bauer M., Bellomo R., Bernard G.R., Chiche J.D., Coopersmith C.M., Hotchkiss R.S., Levy M.M., Marshall J.C., Martin G.S., Opal S.M., Rubenfeld G.D., van der Poll T., Vincent J.L., Angus D.C. The Third International Consensus defnitions for sepsis and septic shock (Sepsis-3). JAMA, 2016, Vol. 315, no. 8, pp. 801-810.
- Vincent J.L., Moreno R., Takala J., Willatts S., De Mendonça A., Bruining H., Reinhart C.K., Suter P.M., Thijs L.G. The SOFA (Sepsis-related Organ Failure Assessment) score to describe organ dysfunction/failure. On behalf of the Working Group on Sepsis-Related Problems of the European Society of Intensive Care Medicine. Intensive Care Med, 1996, Vol. 22, no. 7, pp. 707-710.
- Wiersinga W.J., van der Poll T. Immunopathophysiology of human sepsis. EBioMedicine, 2022, Vol. 86, 104363. doi: 10.1016/j.ebiom.2022.104363.
- Zhang H., Wang Y., Qu M., Li W., Wu D., Cata J.P., Miao C. Neutrophil, neutrophil extracellular traps and endothelial cell dysfunction in sepsis. Clin. Transl. Med., 2023, Vol. 13, no. 1, e1170. doi: 10.1002/ctm2.1170.
补充文件
