MOLECULAR CLONING OF HUMAN THYROID STIMULATING HORMONE RECEPTOR GENE
- 作者: Zubkov A.V.1, Andreeva M.A.1, Sidorov A.V.1, Milovanova A.V.1, Butova L.G.1
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隶属关系:
- Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
- 期: 卷 22, 编号 2-1 (2019)
- 页面: 278-280
- 栏目: ORIGINAL ARTICLES
- URL: https://journal-vniispk.ru/1028-7221/article/view/119763
- DOI: https://doi.org/10.31857/S102872210006600-5
- ID: 119763
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详细
Producing of recombinant TSHR fragments opens up new possibilities in the development of immunochemical tests to detect stimulating and blocking antibodies to TSHR, in studying the role of the antigenic structure of TSHR in the pathogenesis of autoimmune diseases of the thyroid gland. The original mRNA sequence of the α-subunit of the thyroid stimulating hormone receptor, not containing nucleotide substitutions, was cloned. The obtained recombinant vector pVAX1-TSHR, prepared for transfection into cells of mammals and for the expression of the recombinant protein of the α-subunit of the thyroid stimulating hormone receptor.
作者简介
A. Zubkov
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
编辑信件的主要联系方式.
Email: alex_zubkov@list.ru
M. D. PhD, Head of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
俄罗斯联邦M. Andreeva
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
junior researcher, of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
俄罗斯联邦A. Sidorov
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
Ph. D. Head of the Laboratory for Genetics of DNA viruses,
Moscow
俄罗斯联邦A. Milovanova
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
researcher of the Laboratory for Genetics of DNA viruses,
Moscow
俄罗斯联邦L. Butova
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
Ph.D., leading researcher, of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
俄罗斯联邦参考
- Smith T. J. TSHR as a therapeutic target in Graves’ disease. Expert Opin Ther Targets. 2017; 21(4): 427– 432.
- Rapoport B., McLachlan S. M. TSH receptor cleavage into subunits and shedding of the A-subunit; a molecular and clinical perspective. Endocrine Reviews. 2016; 37(2): 114–34.
- Sanders J., Bolton J., Sanders P., Jeff reys J., Nakatake N., Richards T., Evans M., Kiddie A., Summerhayes S., Roberts E., Miguel R. N., Furmaniak J., Smith B. R. Eff ects of TSH receptor mutations on binding and biological activity of monoclonal antibodies and TSH. Thyroid. 2007; 17(2): 1195–1206.
- Furmaniak J., Sanders J., Núñez Miguel R., B. Rees Smith. Mechanisms of Action of TSHR Autoantibodies. Horm Metab Res. 2015; 47: 735–752.
- Parmentier M., Libert F., Maenhaut C., Lefort A., Gerard C., Perret J., Van Sande J., Dumont J. E., Vassart G. Molecular cloning of the thyrotropin receptor. Science. 1989; 246(4937): 1620–1622.
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