Screening of immune status in patients with acute coronary syndrome
- 作者: Safronova E.A.1,2, Ryabova L.V.1, Sarapultsev G.P.3
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隶属关系:
- South-Ural State Medical University
- University of Innovation and Continuing Education, Burnazyan Federal Medical Biophysical Center, Federal Medical-Biological Agency
- Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences
- 期: 卷 28, 编号 3 (2025)
- 页面: 823-828
- 栏目: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/1028-7221/article/view/319941
- DOI: https://doi.org/10.46235/1028-7221-17174-SOI
- ID: 319941
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The aim of the work was to assess differences in clinical pattern and immunological status in patients with acute coronary syndrome (ACS) and Post-COVID syndrome (PCS), taking into account the level of TREC in the peripheral blood. A total of 32 patients aged 40 to 65 years with ACS and a history of COVID-19 from 6 to 18 months were examined. All patients underwent coronary angiography and stenting of the coronary arteries. To determine TREC levels in peripheral blood, the TREC/KREC-AMP PS reagent kit (Pasteur Research Institute, St. Petersburg) was used. To determine the studied subpopulations of peripheral blood lymphocytes, Beckman Coulter (USA) reagents – TetraChrome were used. The analysis of samples was studied with a Navios™ flow cytometer (Beckman Coulter, USA). Among patients with ACS and PCS, the TREC content was determined in 32 patients, including 28 with unstable angina and 3 with acute myocardial infarction without ST segment elevation. The patients were divided into groups depending on the TREC content: (1) with a reduced TREC level (1st group) and with normal index values (2nd group). One should note that only one patient from this group had reduced KREC values, the rest being within normal range. Therefore, we did not find any effect of KREC on clinical and immunological features of subjects with ACS and SCS. Individuals with reduced TREC levels had a lower relative and absolute number of T-helper cells (p < 0.01), and their immunoregulatory index was lower (p < 0.01). At the same time, an increase in relative (p < 0.01) and absolute numbers of T cytotoxic cells (p < 0.05), as well as percentage of T-NK lymphocytes (p < 0.05) was noted in this group of patients. Also, in patients from the 1st group, we have seen a tendency to increase in NK lymphocyte numbers and decrease in B lymphocytes (CD3-CD19+). A low-TREC subgroup of patients with ACS and PCS showed a more severe clinical course. However, due to limited group of patients, further study of such relations is planned with a larger set of clinical cases. Detection of immune disorders using the mentioned screening methods requires novel approaches to immunocorrection of these disorders which are currently discussed by cardiologists.
作者简介
E. Safronova
South-Ural State Medical University; University of Innovation and Continuing Education, Burnazyan Federal Medical Biophysical Center, Federal Medical-Biological Agency
编辑信件的主要联系方式.
Email: safronovaeleonora68@gmail.com
PhD (Medicine), Associate Professor, Department of Polyclinic Therapy and Clinical Pharmacology, Lecturer, Department of Therapy
俄罗斯联邦, Chelyabinsk; MoscowL. Ryabova
South-Ural State Medical University
Email: safronovaeleonora68@gmail.com
PhD, MD (Medicine), Associate Professor, Professor, Department of Life Safety, Disaster Medicine, Emergency Medicine
俄罗斯联邦, ChelyabinskG. Sarapultsev
Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences
Email: safronovaeleonora68@gmail.com
Postgraduate Student
俄罗斯联邦, Ekaterinburg参考
- Зурочка А.В., Добрынина М.А., Сафронова Э.А., Зурочка В.А., Зуйкова А.А., Сарапульцев Г.П., Забков О.И., Мосунов А.А., Верховская М.Д., Дукардт В.В., Фомина Л.О., Костоломова Е.Г., Останкова Ю.В., Кудрявцев И.В., Тотолян А.А. Нарушения Т-клеточного звена иммунитета через 6-12 месяцев после острой фазы коронавирусной инфекции: скрининговое исследование // Инфекция и иммунитет, 2024. Т. 14, № 4. С. 756-768. [Zurochka A.V., Dobrynina M.A., Safronova E.A., Zurochka V.A., Zuykova A.A., Sarapultsev G.P., Zabkov O.I., Mosunov A.A., Verkhovskaya M.D., Dukardt V.V., Fomina L.O., Kostolomova E.G., Ostankova Yu.V., Kudryavtsev I.V., Totolyan A.A. Disturbances in the T-cell component of immunity 6-12 months after the acute phase of coronavirus infection: a screening study. Infektsiya i immunitet = Russion Journal of Infection and Immunity, 2024, Vol. 14, no. 4, pp. 756-768. (In Russ.)] doi: 10.15789/2220-7619-AIT-17646.
- Зурочка А.В. Хайдуков С.В., Кудрявцев И.В., Черешнев В.А. Проточная цитометрия в биомедицинских исследованиях. Екатеринбург: РИО УрО РАН, 2018. 720 с. [Zurochka A.V., Khaidukov S.V., Kudryavtsev I.V., Chereshnev V.A. Flow cytometry in biomedical research]. Ekaterinburg: RIO UB RAS, 2018. 720 p.
- Останкова Ю.В., Сайтгалина М.А., Арсентьева Н.А., Тотолян А.А. Оценка уровней TREC/KREC у ВИЧ-инфицированных лиц // ВИЧ-инфекция и иммуносупрессии, 2024. Т. 16, № 2. С. 51-59. [Ostankova Yu.V., Saitgalina M.A., Arsentieva N.A., Totolian A.A. Evaluation of TREC/KREC levels in HIV-infected individuals. VICH-infektsiya i immunosupressii = HIV Infection and Immunosuppressive Disorders, 2024, Vol. 16, no. 2, pp. 51-59. (In Russ.)]
- Сайтгалина М.А., Любимова Н.Е., Останкова Ю.В., кузнецова Р.Н., Тотолян А.А.Определение референтных интервалов циркулирующих в крови эксцизионных колец TREC и KREC у лиц старше 18 лет // Медицинская иммунология, 2022. Т. 24, № 6. С. 1227-1236. [Saytgalina M.A., Lyubimova N.E., Ostankova Yu.V., Kuznetsova R.N., To-tolian A.A. Determination of reference intervals of circulating TREC and KREC excision rings in individuals over 18 years of age. Meditsinskaya immunologiya = Medical Immunology (Russia), 2022, Vol. 24, no. 6, pp. 1227-1236. (In Russ.)] doi: 10.15789/1563-0625-DOR-2587.
- Сайтгалина М.А., Останкова Ю.В., Арсентьева Н.А., Коробова З.Р., Любимова Н.Е., Кащенко В.А., Куликов А.Н., Певцов Д.Э., Станевич О.В., Черных Е.И., Тотолян А.А. Значимость определения уровней молекул TREC и KREC в периферической крови для прогноза исхода заболевания COVID-19 в острый период // Российский иммунологический журнал, 2023. Т. 26, № 4. С. 611-618. [Saytgalina M.A., Ostankova Yu.V., Arsentyeva N.A., Korobova Z.R., Lyubimova N.E., Kashchenko V.A., Kulikov A.N., Pevtsov D.E., Stanevich O.V., Chernykh E.I., Totolyan A.A. The significance of determining the levels of TREC and KREC molecules in peripheral blood for predicting the outcome of COVID-19 disease in the acute period. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2023, Vol. 26, no. 4, pp. 611-618. (In Russ.)] doi: 10.46235/1028-7221-14714-LOT.
- Сайтгалина М.А., Останкова Ю.В., Любимова Н.Е., Семенов А.В., Кузнецова Р.Н., Тотолян А.А. Модифицированный метод количественного определения уровней TREC и KREC в периферической крови у больных с иммунодефицитными состояниями // Инфекция и иммунитет, 2022. Т. 12, № 5. C. 981-996. [Saytgalina M.A., Ostankova Yu.V., Lyubimova N.E., Semenov A.V., Kuznetsova R.N., Totolyan A.A. Modified method for quantitative determination of TREC and KREC levels in peripheral blood in patients with immunodeficiency states. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2022, Vol. 12, no. 5, pp. 981-996. (In Russ.)] doi: 10.15789/2220-7619-MMF-2039.
- Сафронова Э.А., Рябова Л.В. Особенности Т-клеточного звена иммунитета у больных с острым коронарным синдромом, болевших и не болевших COVID-19, в зависимости от содержания натуральных киллеров // Российский иммунологический журнал, 2023. Т. 26, № 3. С. 389-396. [Safronova E.A., Ryabova L.V. Features of the T-cell link of immunity in patients with acute coronary syndrome, who had and did not have COVID-19, depending on the content of natural killers. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2023, Vol. 26, no. 3, pp. 389-396. (In Russ.)] doi: 10.46235/1028-7221-9640-FOT.
- Хайдуков С.В., Байдун Л.А., Зурочка А.В., Тотолян А.А. Стандартизованная технология «Исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов» // Российский иммунологический журнал, 2014. Т. 8 (17), № 4. С. 974-992. [Khaidukov S.V., Baidun L.A., Zurochka A.V., Totolyan A.A. Standardized technology “Study of the subpopulation composition of peripheral blood lymphocytes using flow cytofluorimeter analyzers”. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2014, Vol. 8 (17), no. 4, pp. 974-992. (In Russ.)]
- Greca E., Kacimi O., Poudel S., Wireko A.A., Abdul-Rahman T., Michel G., Marzban S., Michel J. Immunomodulatory effect of different statin regimens on regulatory T-cells in patients with acute coronary syndrome: a systematic review and network meta-analysis of randomized clinical trials. Eur. Heart J. Cardiovasc. Pharmacother., 2023, Vol. 9, no. 2, pp. 122-128.
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