Email this article Measuring Fluctuations in the Intensity of a Single Point-Like Luminescence Emitter: Artifacts in Processing Microscopic Images
- Authors: Eremchev I.Y.1,2, Lozing N.A.1,3, Gladush M.G.1,2,4, Bayev A.A.5, Rozhentsov A.A.5, Naumov A.V.1,2,4
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Affiliations:
- Institute of Spectroscopy, Russian Academy of Sciences
- Moscow Institute of Physics and Technology (State University)
- National Research University Higher School of Economics
- Moscow Pedagogical State University
- Volga State Technological University
- Issue: Vol 82, No 11 (2018)
- Pages: 1482-1486
- Section: Article
- URL: https://journal-vniispk.ru/1062-8738/article/view/186840
- DOI: https://doi.org/10.3103/S1062873818080166
- ID: 186840
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Abstract
The processing of images of point-like fluorescing objects, obtained by a matrix detector in the scheme of a luminescent microscope, is considered. Analysis of model images with parameters close to experimental images of flickering single CdSe/ZnS quantum dots is presented. A scheme for obtaining information on fluctuations in fluorescence intensity is proposed.
About the authors
I. Yu. Eremchev
Institute of Spectroscopy, Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)
Author for correspondence.
Email: eremchev@isan.troitsk.ru
Russian Federation, Troitsk, Moscow oblast, 108840; Moscow, 141701
N. A. Lozing
Institute of Spectroscopy, Russian Academy of Sciences; National Research University Higher School of Economics
Email: eremchev@isan.troitsk.ru
Russian Federation, Troitsk, Moscow oblast, 108840; Moscow, 101000
M. G. Gladush
Institute of Spectroscopy, Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University); Moscow Pedagogical State University
Email: eremchev@isan.troitsk.ru
Russian Federation, Troitsk, Moscow oblast, 108840; Moscow, 141701; Moscow, 119991
A. A. Bayev
Volga State Technological University
Email: eremchev@isan.troitsk.ru
Russian Federation, Yoshkar-Ola
A. A. Rozhentsov
Volga State Technological University
Email: eremchev@isan.troitsk.ru
Russian Federation, Yoshkar-Ola
A. V. Naumov
Institute of Spectroscopy, Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University); Moscow Pedagogical State University
Email: eremchev@isan.troitsk.ru
Russian Federation, Troitsk, Moscow oblast, 108840; Moscow, 141701; Moscow, 119991
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