Cultivated Cells of Mytilus trossulus Gould, 1850: The Effect of Cold Stress

Abstract

This study continues our previous work on the pathways of the death of marine invertebrate cells in response to cold stress. An analysis of changes in cells of the mussel Mytilus trossulus after cold stress that was carried out using various methods can be the key to revealing the mechanisms of adaptation of marine hydrobionts to changes in environmental conditions. Unfortunately, using tested apoptosis inhibitors, we could not produce either a decrease in apoptosis or an increase in cell viability in primary larval cell cultures of this bivalve mollusk after a freeze–thaw cycle. Apoptosis is probably rare in these cell cultures, or mollusks possess a more powerful anti-apoptotic system than that of mammals.