Email this article Small-angle X-ray scattering study of the influence of solvent replacement (from H2O to D2O) on the initial crystallization stage of tetragonal lysozyme
- Authors: Boikova A.S.1,2, D’yakova Y.A.1,2, Il’ina K.B.1,2, Konarev P.V.1,2, Kryukova A.E.1,2, Marchenkova M.A.1,2, Blagov A.E.1,2, Pisarevskii Y.V.1,2, Koval’chuk M.V.1,2,3
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Affiliations:
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
- National Research Centre “Kurchatov Institute,”
- St. Petersburg State University
- Issue: Vol 62, No 6 (2017)
- Pages: 837-842
- Section: Diffraction and Scattering of Ionizing Radiations
- URL: https://journal-vniispk.ru/1063-7745/article/view/191407
- DOI: https://doi.org/10.1134/S1063774517060074
- ID: 191407
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Abstract
The composition of lysozyme solutions in D2O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with H2O used as a solvent has shown that the replacement of light water with heavy one leads to increase of octamer volume fraction in solution.
About the authors
A. S. Boikova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Author for correspondence.
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
Yu. A. D’yakova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
K. B. Il’ina
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
P. V. Konarev
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
A. E. Kryukova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
M. A. Marchenkova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
A. E. Blagov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
Yu. V. Pisarevskii
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
M. V. Koval’chuk
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; National Research Centre “Kurchatov Institute,”; St. Petersburg State University
Email: boikova_as@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182; St. Petersburg, 199034
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