Email this article Microfluidic Cell for Studying the Precrystallization Stage Structure of Protein Solutions by Small-Angle X-Ray Scattering
- Authors: Popov A.M.1, Boikova A.S.1,2, Volkov V.V.2, D’yakova Y.A.1,2, Il’ina K.B.1,2, Konarev P.V.1,2, Marchenkova M.A.1,2, Peters G.S.1, Pisarevskii Y.V.1,2, Koval’chuk M.V.1,2,3
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Affiliations:
- National Research Centre “Kurchatov Institute,”
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
- St. Petersburg State University
- Issue: Vol 63, No 5 (2018)
- Pages: 713-718
- Section: Diffraction and Scattering of Ionizing Radiations
- URL: https://journal-vniispk.ru/1063-7745/article/view/192860
- DOI: https://doi.org/10.1134/S1063774518050231
- ID: 192860
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Abstract
A microfluidic cell has been developed for studying the structure of protein solutions using small-angle X-ray scattering. The use of prefabricated elements, which provide a small sample volume, allows one to adjust the microfluidic cell parameters to specific problems. Tests of lysozyme solutions were performed on a laboratory diffractometer AMUR-K (Institute of Crystallography, Russian Academy of Sciences), BM29 beamline (ESRF, Grenoble), and “DICSY” beamline of the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”). Experiments showed that, when a 60-μm-thick quartz glass of special purity grade is applied as an X-ray-transparent window, the intrinsic level of cell background scattering in the range of measured angles is lower than that for quartz capillaries, due to which the measurement accuracy can be increased.
About the authors
A. M. Popov
National Research Centre “Kurchatov Institute,”
Author for correspondence.
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182
A. S. Boikova
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333
V. V. Volkov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 119333
Yu. A. D’yakova
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333
K. B. Il’ina
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333
P. V. Konarev
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333
M. A. Marchenkova
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333
G. S. Peters
National Research Centre “Kurchatov Institute,”
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182
Yu. V. Pisarevskii
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333
M. V. Koval’chuk
National Research Centre “Kurchatov Institute,”; Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences; St. Petersburg State University
Email: antmp.86@yandex.ru
Russian Federation, Moscow, 123182; Moscow, 119333; St. Petersburg, 199034
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