Email this article Comparative Analysis of Different Methods of Scanning Electron Microscopy and Test Preparation in Biological Tissue Studies
- Authors: Kamyshinskii R.A.1,2,3, Orekhov A.S.1,2, Grigor’ev T.E.1, Kuevda E.V.4, Gubareva E.A.4, Tokaev K.V.5, Chvalun S.N.1, Vasil’ev A.L.1,2,3
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Affiliations:
- National Research Centre “Kurchatov Institute”
- Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- Moscow Institute of Physics and Technology (State University)
- Kuban State Medical University, Ministry of Health of the Russian Federation
- National Research Medical Center of Phthisiopulmonology and Infection Diseases, Ministry of Health of the Russian Federation
- Issue: Vol 64, No 3 (2019)
- Pages: 466-469
- Section: Nanomaterials and Ceramics
- URL: https://journal-vniispk.ru/1063-7745/article/view/193989
- DOI: https://doi.org/10.1134/S106377451903012X
- ID: 193989
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Abstract
Different methods of scanning electron microscopy in combination with different sample preparation techniques have been compared by an example of decellularized rat diaphragm matrices. It is shown that a combination of scanning electron microscopy under natural environmental conditions, allowing one to investigate tissue samples in a state similar to native, with the conventional high-vacuum microscopy of samples obtained by drying at the critical point, provides the most complete microstructural analysis of native and decellularized tissue samples.
About the authors
R. A. Kamyshinskii
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)
Email: a.vasiliev56@gmail.com
Russian Federation, Moscow, 123182; Moscow, 119333; Dolgoprudny, Moscow oblast, 141701
A. S. Orekhov
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Email: a.vasiliev56@gmail.com
Russian Federation, Moscow, 123182; Moscow, 119333
T. E. Grigor’ev
National Research Centre “Kurchatov Institute”
Email: a.vasiliev56@gmail.com
Russian Federation, Moscow, 123182
E. V. Kuevda
Kuban State Medical University, Ministry of Health of the Russian Federation
Email: a.vasiliev56@gmail.com
Russian Federation, Krasnodar
E. A. Gubareva
Kuban State Medical University, Ministry of Health of the Russian Federation
Email: a.vasiliev56@gmail.com
Russian Federation, Krasnodar
K. V. Tokaev
National Research Medical Center of Phthisiopulmonology and Infection Diseases,Ministry of Health of the Russian Federation
Email: a.vasiliev56@gmail.com
Russian Federation, Moscow
S. N. Chvalun
National Research Centre “Kurchatov Institute”
Email: a.vasiliev56@gmail.com
Russian Federation, Moscow, 123182
A. L. Vasil’ev
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)
Author for correspondence.
Email: a.vasiliev56@gmail.com
Russian Federation, Moscow, 123182; Moscow, 119333; Dolgoprudny, Moscow oblast, 141701
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