Spectral Characteristics of the DMEM Cell-Culture Medium

A. A. Zhikhoreva; V. P. Belik; A. V. Belashov; O. S. Vasyutinskii; I. M. Gadzhiev; S. E. Goncharov; I. V. Semenova

doi:10.1134/S1063784218090256

Spectral Characteristics of the DMEM Cell-Culture Medium

作者: Zhikhoreva A.A.¹^,2, Belik V.P.¹, Belashov A.V.¹, Vasyutinskii O.S.¹, Gadzhiev I.M.¹, Goncharov S.E.¹, Semenova I.V.¹
隶属关系:
1. Ioffe Institute, Russian Academy of Sciences
2. ITMO University
期: 卷 63, 编号 9 (2018)
页面: 1300-1303
栏目: Physical Approaches and Problems of Data Interpretation in the Life Sciences
URL: https://journal-vniispk.ru/1063-7842/article/view/201936
DOI: https://doi.org/10.1134/S1063784218090256
ID: 201936

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详细

Spectral characteristics of luminescence of the DMEM cell-culture medium are studied. The luminescence spectrum of the medium is determined in the visible and near-IR spectral ranges at wavelengths of up to 1350 nm under excitation at 405 and 660 nm. The lifetimes of excited states of the medium are determined, and the absence of singlet oxygen phosphorescence at about 1270 nm is demonstrated.

关键词

Cell Culture Media, Dulbecco’s Modified Eagle’s Medium (DMEM), Singlet Oxygen Phosphorescence, Luminescence Spectra, Fluorescence Lifetime Imaging Microscopy (FLIM)

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