Specific properties of hydroxyapatite as a potential transporter of copper ions and its complexes


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Abstract

Modification of the cocrystallization method for producing hydroxyapatite (HAP) and an HAP-Cu combination to the enzymatic method using alkaline phosphatase leads to a change in the morphology, sizes, sorption capacity, type of particles, and conformity with the Langmuir and Freundlich models. A positive factor of the enzyme usage is an increase in the sorption capacity and the possibility to strictly control the particle sizes depending on the concentration of the enzyme used. The L2CuCl4 complex was synthesized on the basis of 2-aminopyrimidine (L), which is the precursor of many anticancer drugs, and the possibilities of introducing L2CuCl4 into the HAP composite were considered. The cytotoxicity data for various HAP and L2CuCl4 composites with respect to various types of leukemic cells as compared to lymphocytes of healthy donors showed antileukemic activity of the copper complex and the absence of HAP cytotoxicity in a wide range of concentrations.

About the authors

M. A. Orlova

Chemical Department, M. V. Lomonosov Moscow State University; Department of Biochemistry and Pharmacology, Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology; Medical Faculty, N. I. Pirogov Russian National Research Medical University

Author for correspondence.
Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991; 1 ul. Samory Mashela, Moscow, 117997; 1 ul. Ostrovityanova, Moscow, 117997

A. L. Nikolaev

Chemical Department, M. V. Lomonosov Moscow State University

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991

T. P. Trofimova

Chemical Department, M. V. Lomonosov Moscow State University; Institute of Physiologically Active Compounds, Russian Academy of Sciences

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991; 1 Severnyi proezd, Chernogolovka, Moscow Region, 142432

A. V. Severin

Chemical Department, M. V. Lomonosov Moscow State University

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991

A. V. Gopin

Chemical Department, M. V. Lomonosov Moscow State University

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991

N. S. Zolotova

Chemical Department, M. V. Lomonosov Moscow State University

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991

V. K. Dolgova

Chemical Department, M. V. Lomonosov Moscow State University

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991

A. P. Orlov

Chemical Department, M. V. Lomonosov Moscow State University

Email: orlova.radiochem@mail.ru
Russian Federation, Building 3, 1 Leninskie Gory, Moscow, 119991

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