Biocatalytic properties of immobilized inulinase

Abstract

A heterogeneous biocatalyst based on inulinase immobilized on a nonionic sorbent of Stirosorb series was proposed. Thermal and acid inactivation of free and immobilized inulinase was examined and the corresponding inactivation constants were calculated. An increase in the thermal stability of the immobilized enzyme in comparison to the free one was found. The possibility of using the immobilized enzyme in the hydrolysis of inulin for ten cycles was determined.