Cyanine-dye-modified 2′-deoxyuridine-5′-triphosphates: Synthesis, applications, and linker effect on substrate properties for Taq DNA polymerase

M. A. Spitsyn; V. E. Kuznetsova; V. E. Shershov; S. A. Lapa; T. O. Guseinov; A. S. Zasedatelev; A. V. Chudinov

doi:10.1134/S1068162017040136

Cyanine-dye-modified 2′-deoxyuridine-5′-triphosphates: Synthesis, applications, and linker effect on substrate properties for Taq DNA polymerase

Authors: Spitsyn M.A.¹, Kuznetsova V.E.¹, Shershov V.E.¹, Lapa S.A.¹, Guseinov T.O.¹, Zasedatelev A.S.¹, Chudinov A.V.¹
Affiliations:
1. Engelhardt Institute of Molecular Biology
Issue: Vol 43, No 4 (2017)
Pages: 471-475
Section: Letter to the Editor
URL: https://journal-vniispk.ru/1068-1620/article/view/228629
DOI: https://doi.org/10.1134/S1068162017040136
ID: 228629

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Abstract

A number of fluorescently labeled nucleoside triphosphates containing electroneutral indodicarbocyanine dye (Cy) have been synthesized. The dye has been attached to the C5 position of pyrimidine through the transalkene linkers of different structure. The synthesized labeled nucleoside triphosphates have been tested as substrates for Taq polymerase in PCR using the “TB-biochip” test system.