Development of Technology for the Preparation of Zinc-Dihydroquercetin Complex

Abstract

The urgency of developing the technology for obtaining the dihydroquercetin-zinc complex is due to the high antiradical, antioxidant, antiviral activity and wound healing effects of the original pharmaceutical composition developed on its basis. The study aims to optimize the complexation of dihydroquercetin (DHQ) with Zn²⁺ ions in aqueous solution and to develop a technological scheme for preparation of the complex. Previously, we have determined the reaction parameters for the laboratory scheme for preparing the complex. A tenfold increase in the volume of the reaction solution (from 50 to 500 mL) has led to a decrease in the yield of the product. The task was to correct some reaction parameters for increasing the yield of the complex. It has been found that the increase in the reaction time (from 15 to 240 min) and the DHQ concentration in the reaction solution (from 0.05 to 0.1 M), while maintaining the previously optimized reaction parameters (the molar ratio Zn²⁺: DKB = 2: 1, pH 5.6), leads to an increase in the yield of the complex from 12 to 84%. Correspondence between the structures of the complex and the compound previously obtained in small volumes has been confirmed by the data of IR, ¹³C and ¹H NMR spectroscopy and elemental analysis. The study will contribute to the more intensive use of extractive substances of larch wood to create new drugs that have antioxidant properties intended for the treatment of various diseases.