Отправить статью по E-mail Novel 5-Alkylcarboxamide-2'-Deoxyuridine-5'-Triphosphates for Enzymatic Synthesis of Highly Modified DNA
- Авторы: Spitsyn M.A.1,2, Guseinov T.O.1,2, Miftahov R.A.1,2, Zasedateleva O.A.1, Lisitsa A.V.3, Radko S.P.2,3, Zasedatelev A.S.1, Timofeev E.N.1, Chudinov A.V.1,2, Vasiliskov V.A.1, Lapa S.A.1, Kuznetsova V.E.1, Surzhikov S.A.1, Shershov V.E.1
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Учреждения:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- IBMС-EcoBioPharm
- Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences
- Выпуск: Том 45, № 3 (2019)
- Страницы: 221-223
- Раздел: Letter to Editor
- URL: https://journal-vniispk.ru/1068-1620/article/view/229198
- DOI: https://doi.org/10.1134/S1068162019030063
- ID: 229198
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Аннотация
The synthesis of deoxyuridine triphosphate derivatives with alkyl groups of various chain length attached to the pyrimidine base by the trans-alkene linker was performed. The efficiency of the incorporation of modified nucleotides during the synthesis of DNA with a high degree of modification by the primer extension reaction with Taq DNA polymerase was studied.
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Об авторах
M. Spitsyn
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Email: chud@eimb.ru
Россия, Moscow, 119991; Moscow, 119121
T. Guseinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Email: chud@eimb.ru
Россия, Moscow, 119991; Moscow, 119121
R. Miftahov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Email: chud@eimb.ru
Россия, Moscow, 119991; Moscow, 119121
O. Zasedateleva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
A. Lisitsa
Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119121
S. Radko
IBMС-EcoBioPharm; Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119121; Moscow, 119121
A. Zasedatelev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
E. Timofeev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
A. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Автор, ответственный за переписку.
Email: chud@eimb.ru
Россия, Moscow, 119991; Moscow, 119121
V. Vasiliskov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
S. Lapa
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
V. Kuznetsova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
S. Surzhikov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
V. Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Россия, Moscow, 119991
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