Obtaining of transgenic black medic plants with the pea defensin gene

Abstract

To assess the potential use of pea defensin gene for protection from phytopathogens, some independent transgenic lines Medicago lupulina L. cv. Mir were obtained by agrobacterial transformation. Regenerants and their seed progeny were analyzed by PCR. To evaluate the potential resistance of the seed progeny selected by field experience (T1, which confirmed the presence of the target transgene by PCR), the mixture of cultural filtrate of highly virulent isolates of Fusarium (F. culmorum, F. sambucinum, F. oxysporum, F. avenaceum, F. gibbosum) was used. An increase in resistance of the studied transgenic lines was demonstrated to the toxic effects of Fusarium damage.