In vitro study of complexing properties of high-molecular water-soluble polymer in relation to model protein

Resumo

The objective of this paper was to study in vitro the complexing ability of anionic polyelectrolyte in relation to model protein to develop a new type of polymeric feed additive for agricultural animals. It has been established that the investigated polymer with a molecular weight of 1 × 10⁶ forms interpolymer complexes with model protein, namely, bovine serum albumin (BSA). The degree of the formation of these complexes significantly depends on the acidity of the medium and the ratio of components. It has been established that these complexes are formed in a wide interval of the ratio of components, i.e., both in the excess of protein in relation to polymer and in the excess of polymer in relation to protein. It has been revealed that 2.0–2.5% of BSA is complexed under conditions that are similar to the conditions of ruminant forestomachs at reaction medium pH 5.0–6.5 and a polymer-to-protein weight ratio of approximately 0.3 × 10^–3.