Antiviral activity of the drug based on the complex of single-stranded high-polymer RNA against the rabies virus in vitro
- Authors: Generalov S.V.1, Perevoznikov D.A.1, Abramova Е.G.1,2, Nikiforov A.K.1,2, Spitsyn A.A.3, Potupchik T.V.4, Shenderovich Е.M.5
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Affiliations:
- Russian Anti-Plague Research Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
- Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
- Limited Liability Company «SPA Evolution of Nature»
- Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University of the Ministry of Health of the Russian Federation
- The Gamaleya National Center of Epidemiology and Microbiology
- Issue: Vol 28, No 8 (2025)
- Pages: 38-44
- Section: Biological chemistry
- URL: https://journal-vniispk.ru/1560-9596/article/view/310627
- DOI: https://doi.org/10.29296/25877313-2025-08-05
- ID: 310627
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Abstract
Introduction. Rabies is an infectious disease with absolute mortality. Currently, there are no effective drugs and treatment regimens for people with signs of rabies. The only strategy for post-exposure rabies prevention is vaccination and administration of rabies immunoglobulin. At the same time, the search for new treatment and prevention regimens for the disease remains relevant, which is why the development and study of antiviral drugs effective against the rabies virus is relevant.
Objective of the study. To study the antiviral activity of a drug based on single-stranded high-polymer RNA of Sacharomyces cerevisiae, which has previously shown antiviral activity against other pathogens of viral infections.
Material and methods. The work investigated the effect of the drug based on single-stranded high-polymer RNA of S. cerevisiae "Amphievovir" on the level of reproduction of the attenuated rabies virus strain "Saratov" on the Vero cell culture model.
Results. The studied drug in the concentration range from 10 to 20 μg/ml and added to the cell culture no later than 24 hours after infection reduces the titer of the virus by 24.6 to 36.2% compared to the control group. Adding the drug to the infected cell culture later than 24 hours reduces its antiviral effect.
Conclusions. The studied drug based on single-stranded high-polymer RNA of S. cerevisiae has antiviral activity against the rabies virus in vitro. The results allow us to justify further study of post-exposure rabies prophylaxis regimens using drugs based on immunostimulating and immunomodulatory RNA.
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##article.viewOnOriginalSite##About the authors
S. V. Generalov
Russian Anti-Plague Research Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Author for correspondence.
Email: svgeneraloff@gmail.com
ORCID iD: 0000-0003-1461-5383
SPIN-code: 4130-2475
Ph.D. (Biol.), Leading Research Scientist, Laboratory of Preventive Immunoglobulins
Russian Federation, Universitetskaya St., 46, Saratov, 410005D. A. Perevoznikov
Russian Anti-Plague Research Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: rusrapi@microbe.ru
Master's Student, Laboratory Assistant-Researcher, Laboratory of Preventive Immunoglobulins
Russian Federation, Universitetskaya St., 46, Saratov, 410005Е. G. Abramova
Russian Anti-Plague Research Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
Email: rusrapi@microbe.ru
ORCID iD: 0000-0002-8798-1547
SPIN-code: 3382-3399
Dr.Sc. (Biol.), Head of the Laboratory of Preventive Immunoglobulins
Russian Federation, Universitetskaya St., 46, Saratov, 410005; Pyotr Stolypin Avenue, building 4, building 3, Saratov, 410012A. K. Nikiforov
Russian Anti-Plague Research Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
Email: rusrapi@microbe.ru
ORCID iD: 0000-0002-1130-3504
SPIN-code: 3202-3979
Dr.Sc. (Biol.), Professor, Deputy Director for Experimental and Production Work
Russian Federation, Universitetskaya St., 46, Saratov, 410005; Pyotr Stolypin Avenue, building 4, building 3, Saratov, 410012A. A. Spitsyn
Limited Liability Company «SPA Evolution of Nature»
Email: evoprirody@yandex.ru
ORCID iD: 0009-0005-4900-826X
Chief Research Scientist
Russian Federation, Voskhod St. 20/1, room 1.2, Novosibirsk, 630102T. V. Potupchik
Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University of the Ministry of Health of the Russian Federation
Email: potupchik_tatyana@mail.ru
ORCID iD: 0000-0003-1133-4447
SPIN-code: 8353-3513
Ph.D. (Med.), Associate Professor of the Department of Pharmacology and Clinical Pharmacology with a postgraduate course
Russian Federation, 1, Partizan Zheleznyak str., Krasnoyarsk, 660022Е. M. Shenderovich
The Gamaleya National Center of Epidemiology and Microbiology
Email: rusrapi@microbe.ru
ORCID iD: 0009-0003-3382-1411
Laboratory Research Assistant, Laboratory of Indication and Ultrastructural Analysis of Microorganisms
Russian Federation, Gamaleyi street, building 18, Moscow, 123098References
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