Email this article Mathematics analysis of polymerase chain reaction kinetic curves
- Authors: Sochivko D.G.1, Fedorov A.A.2, Varlamov D.A.3, Kurochkin V.E.2, Petrov R.V.4
-
Affiliations:
- JSC Syntol
- Institute for Analytical Instrumentation
- All-Russia Research Institute of Agricultural Biotechnology
- National Research Center, Institute of Immunology
- Issue: Vol 466, No 1 (2016)
- Pages: 13-16
- Section: Biochemistry, Biophysics and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/210975
- DOI: https://doi.org/10.1134/S160767291601004X
- ID: 210975
Cite item
Open Access
Access granted
Subscription Access
Abstract
The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. Approximation of PCR kinetic curves and PCR efficiency curves by various functions is described. Several PCR models based on chemical kinetics equations are suggested. Decision criteria for an optimal function to describe PCR efficiency are proposed.
About the authors
D. G. Sochivko
JSC Syntol
Email: faa@bk.ru
Russian Federation, ul. Timiryazevskaya 42, Moscow, 127550
A. A. Fedorov
Institute for Analytical Instrumentation
Author for correspondence.
Email: faa@bk.ru
Russian Federation, Rizhskii pr. 26, St. Petersburg, 190103
D. A. Varlamov
All-Russia Research Institute of Agricultural Biotechnology
Email: faa@bk.ru
Russian Federation, ul. Timiryazevskaya 42, Moscow, 127550
V. E. Kurochkin
Institute for Analytical Instrumentation
Email: faa@bk.ru
Russian Federation, Rizhskii pr. 26, St. Petersburg, 190103
R. V. Petrov
National Research Center, Institute of Immunology
Email: faa@bk.ru
Russian Federation, Kashirskoe sh. 24, Moscow, 115478
Supplementary files
