Email this article Whole genome SNP scanning of snow sheep (Ovis nivicola)
- Authors: Deniskova T.E.1, Okhlopkov I.M.1,2, Sermyagin A.A.1, Gladyr’ E.A.1, Bagirov V.A.1, Sölkner J.3, Mamaev N.V.2, Brem G.1,4, Zinov’eva N.A.1
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Affiliations:
- Biotechnology and Molecular Diagnostics Center
- Institute of Biological Problems of Cryolithozone
- Division of Livestock Sciences
- Institute of Animal Breeding and Genetics
- Issue: Vol 469, No 1 (2016)
- Pages: 288-293
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/211451
- DOI: https://doi.org/10.1134/S1607672916040141
- ID: 211451
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Abstract
This is the first report performing the whole genome SNP scanning of snow sheep (Ovis nivicola). Samples of snow sheep (n = 18) collected in six different regions of the Republic of Sakha (Yakutia) from 64° to 71° N. For SNP genotyping, we applied Ovine 50K SNP BeadChip (Illumina, United States), designed for domestic sheep. The total number of genotyped SNPs (call rate 90%) was 47796 (88.1% of total SNPs), wherein 1006 SNPs were polymorphic (2.1%). Principal component analysis (PCA) showed the clear differentiation within the species O. nivicola: studied individuals were distributed among five distinct arrays corresponding to the geographical locations of sampling points. Our results demonstrate that the DNA chip designed for domestic sheep can be successfully used to study the allele pool and the genetic structure of snow sheep populations.
About the authors
T. E. Deniskova
Biotechnology and Molecular Diagnostics Center
Author for correspondence.
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132
I. M. Okhlopkov
Biotechnology and Molecular Diagnostics Center; Institute of Biological Problems of Cryolithozone
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132; ul. Lenina 41, Yakutsk, 677980
A. A. Sermyagin
Biotechnology and Molecular Diagnostics Center
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132
E. A. Gladyr’
Biotechnology and Molecular Diagnostics Center
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132
V. A. Bagirov
Biotechnology and Molecular Diagnostics Center
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132
J. Sölkner
Division of Livestock Sciences
Email: horarka@yandex.ru
Austria, Augasse 2-6, Vienna, A-1090
N. V. Mamaev
Institute of Biological Problems of Cryolithozone
Email: horarka@yandex.ru
Russian Federation, ul. Lenina 41, Yakutsk, 677980
G. Brem
Biotechnology and Molecular Diagnostics Center; Institute of Animal Breeding and Genetics
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132; Veterinärplatz, Vienna, A-1210
N. A. Zinov’eva
Biotechnology and Molecular Diagnostics Center
Email: horarka@yandex.ru
Russian Federation, Moscow oblast, 142132
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