Expression of the FAP gene in non-fibroblast human cell lines. Development of cancer-associated fibroblast models
- Authors: Tyulkina D.V.1, Pleshkan V.V.1, Alekseenko I.V.1, Kopantseva M.R.1, Sverdlov E.D.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 470, No 1 (2016)
- Pages: 319-321
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/211529
- DOI: https://doi.org/10.1134/S1607672916050033
- ID: 211529
Cite item
Abstract
The fibroblast activation protein (FAP) is selectively expressed in cancer-associated fibroblasts (CAFs) and facilitates tumor progression, which makes this protein an attractive therapeutic target. There are difficulties in obtaining CAFs for studying the function and suppression of FAP. In this work, the expression level of FAP was determined by PCR assay in 25 human cell lines and 8 surgical samples of tumor stroma. The expression of FAP was observed in all tumor stroma samples and in four cell lines: NGP-127, SJCRH30, SJSA-1, and A375. The level of FAP expression in NGP-127, SJCRH30, and SJSA-1 lines as well as in CAFs of patients was comparable, which makes these cell lines a possible model for studying FAP.
About the authors
D. V. Tyulkina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: tyulkina.dina@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
V. V. Pleshkan
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tyulkina.dina@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
I. V. Alekseenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tyulkina.dina@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
M. R. Kopantseva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tyulkina.dina@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
E. D. Sverdlov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tyulkina.dina@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
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